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产品描述：人金属肽酶含血小板反应蛋白1(ADAMTS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTS1含量。ADAMTS1 是重要的靶点。它参与多种生理病理过程，如炎症反应、肿瘤发生发展等。其研究机制主要聚焦在其酶活性对细胞外基质的调控，以及与其他信号通路的相互作用，这些研究有助于深入理解疾病机制和开发新的治疗策略。试剂盒检测范围为0.312 ng/mL-20ng/mL，适用于体外研究ADAMTS1在心血管疾病、肿瘤转移或组织纤维化等领域的表达调控机制，也可用于评估疾病模型动物或细胞培养体系中该蛋白的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A disintegrin and metalloproteinase with thrombospondin motifs 1 ELISA Kit; ADAM-TS 1 ELISA Kit; ADAM-TS1 ELISA Kit; ADAMTS 1 ELISA Kit; ADAMTS ELISA Kit; ADAMTS-1 ELISA Kit; Adamts1 ELISA Kit; ATS1_HUMAN ELISA Kit; C3 C5 ELISA Kit; KIAA1346, ELISA Kit; METH 1 ELISA Kit; METH-1 ELISA Kit
缩写：ADAMTS1
Uniprot No.：Q9UHI8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human ADAMTS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.0852.1522.1191.999 101.4551.4151.4351.315 50.9531.0040.9790.859 2.50.5760.5790.5780.458 1.250.3510.3360.3440.224 0.6250.2480.2680.2580.138 0.3120.1820.1790.1810.061 00.1230.1170.120 | ||||||
货期：3-5 working days
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