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产品描述：小鼠ADAM金属肽酶含血小板反应蛋白1型13(ADAMTS13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTS13含量。ADAMTS13是重要的靶点，它主要作用是裂解血管性血友病因子（VWF）多聚体。当ADAMTS13活性严重缺乏时，超大分子VWF多聚体增多，易引发血小板聚集形成微血栓，进而导致血栓性血小板减少性紫癜等疾病，目前其相关研究也多围绕此机制开展。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于研究血栓性疾病模型、血液系统功能调控、药物干预对 ADAMTS13 表达的影响，或基因编辑动物模型中该蛋白的动态变化分析，为心血管及血液相关领域的机制探索和药物开发提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adamts13 ELISA kit; Gm710A disintegrin and metalloproteinase with thrombospondin motifs 13 ELISA kit; ADAM-TS 13 ELISA kit; ADAM-TS13 ELISA kit; ADAMTS-13 ELISA kit; EC 3.4.24.87 ELISA kit; von Willebrand factor-cleaving protease ELISA kit; vWF-CP ELISA kit; vWF-cleaving protease ELISA kit
缩写：ADAMTS13
Uniprot No.：Q769J6
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAMTS13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| 1:2000 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:4000 | Average % | 92 | ||||
| Range % | 85-95 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse ADAMTS13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-94 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.4012.4762.4392.33725001.8841.8751.8801.77812501.4131.4241.4191.3176250.7770.7980.7880.6863120.4660.4760.4710.3691560.2650.2580.2620.160780.1760.1840.1800.07800.1010.1030.102 | ||||||
货期：3-5 working days
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