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产品描述：小鼠解整合素样金属蛋白酶33(ADAM33)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAM33含量。ADAM33是一种编码解整合素和金属蛋白酶的基因，其表达与哮喘、慢性阻塞性肺疾病和鼻炎等疾病相关。该基因通过参与细胞粘附、迁移和信号传导过程，在多种生物过程中发挥作用，如受精、肌肉发育和神经发生。ADAM33基因的多态性与哮喘等疾病的易感性有关。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础研究中ADAM33的表达水平分析，如探索肺部疾病模型中的分子调控机制、评估药物干预对气道重塑的影响，或验证基因编辑动物模型中靶蛋白的表达变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adam33Disintegrin and metalloproteinase domain-containing protein 33 ELISA kit; ADAM 33 ELISA kit; EC 3.4.24.- ELISA kit
缩写：ADAM33
Uniprot No.：Q923W9
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 99-109 | |||||
回收率：
| The recovery of mouse ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6582.5522.6052.4345001.8812.0061.9441.7732501.1281.2041.1660.9951250.6270.6530.6400.46962.50.3720.4010.3870.21631.20.2760.2640.2700.09915.60.2340.2390.2370.06600.1740.1670.171 | ||||||
货期：3-5 working days
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