首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠解整合素样金属蛋白酶8(ADAM8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAM8含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，试剂盒适用于基础科研领域，例如炎症机制研究、肿瘤微环境分析、呼吸道病理模型评估或药物干预实验中ADAM8的动态监测，为探索其生物学功能及调控网络提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adam8 ELISA kit; Ms2Disintegrin and metalloproteinase domain-containing protein 8 ELISA kit; ADAM 8 ELISA kit; EC 3.4.24.- ELISA kit; Cell surface antigen MS2 ELISA kit; Macrophage cysteine-rich glycoprotein ELISA kit; CD antigen CD156a ELISA kit
缩写：ADAM8
Uniprot No.：Q05910
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-98 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-107 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 82-94 | ||||||
回收率：
| The recovery of mouse ADAM8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 87-97 | |||||
| EDTA plasma (n=4) | 105 | 95-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.2262.3442.2852.17120001.8061.9231.8651.75110001.3411.3541.3481.2345000.7230.7500.7370.6232500.4400.4110.4260.3121250.2780.2870.2830.16962.50.2130.2050.2090.09500.1120.1150.114 | |||||||
货期：3-5 working days
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