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产品描述：人解整合素样金属蛋白酶33(ADAM33)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、唾液、组织匀浆样本中的ADAM33含量。ADAM33 是一种跨膜蛋白，在哮喘发病机制中起重要作用。其参与气道重塑和炎症反应，可促进气道平滑肌细胞增殖和迁移。研究该靶点，有助于深入理解哮喘病理机制、寻找新治疗靶点和开发有效治疗药物。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，支持科研人员开展呼吸疾病分子机制、炎症反应调控或组织修复等基础研究中的靶点表达水平分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A disintegrin and metalloprotease 33 ELISA Kit; A disintegrin and metalloproteinase domain 33 ELISA Kit; ADA33_HUMAN ELISA Kit; ADAM 33 ELISA Kit; ADAM metallopeptidase domain 33 ELISA Kit; ADAM33 ELISA Kit; C20orf153 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 33 ELISA Kit; Disintegrin and reprolysin metalloproteinase family protein ELISA Kit; DJ964F7.1 ELISA Kit; DKFZp434K0521 ELISA Kit; FLJ35308 ELISA Kit; FLJ36751 ELISA Kit; Metalloprotease disintegrin ELISA Kit; MGC149823 ELISA Kit; MGC71889 ELISA Kit; PRO1891 ELISA Kit; UNQ873 ELISA Kit
缩写：ADAM33
Uniprot No.：Q9BZ11
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, saliva, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-93 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7482.6722.7102.58110002.1312.0052.0681.9395001.4911.4861.4891.3602500.9660.9350.9510.8221250.5460.5740.5600.43162.50.3630.3920.3780.24931.250.2540.2430.2490.12000.1250.1320.129 | ||||||
货期：3-5 working days
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