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产品描述：鸡促肾上腺皮质激素(ACTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACTH含量。促肾上腺皮质激素（ACTH）是一种由脑垂体前叶分泌的多肽激素，主要作用是刺激肾上腺皮质分泌皮质醇，参与调节生物体的应激反应和昼夜节律。其分泌受下丘脑促肾上腺皮质激素释放激素（CRH）调控，并通过负反馈机制维持激素水平的平衡。研究ACTH有助于理解内分泌系统的调节机制和疾病诊断。试剂盒检测范围为0.25 pg/mL-50 pg/mL，适用于家禽养殖业中应激生理研究、实验室条件下激素分泌调控机制分析，以及疾病模型中肾上腺 - 垂体轴功能评估等科研场景。可为禽类健康管理、环境适应研究及药理学实验中 ACTH 水平监测提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACTH
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.25 pg/mL-50 pg/mL
灵敏度：0.125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 97-95 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 500.1760.1740.175 12.50.3210.3160.319 40.5340.5140.524 10.8880.8570.873 0.251.4321.3241.378 01.9861.9051.946 | ||||||
货期：3-5 working days
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