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产品描述：人α平滑肌肌动蛋白(α-SMA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的ACTA2含量。ACTA2 是一种重要的靶点。它在维持细胞结构、运动等方面发挥关键作用。研究发现其异常表达与多种疾病相关，如血管疾病、肿瘤等。相关机制研究聚焦于其在细胞信号传导、组织重塑等过程中的功能，为疾病诊断和治疗提供新方向。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于肝/肺纤维化模型研究、肿瘤微环境分析、动脉粥样硬化机制探索等科研领域，能够精准检测多种生物样本中α - SMA的动态变化，为细胞外基质重构、病理纤维化进程及间质细胞活化等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：a actin ELISA Kit; AAT6 ELISA Kit; ACTA_HUMAN ELISA Kit; ACTA2 ELISA Kit; Actin alpha 2 smooth muscle aorta ELISA Kit; Actin aortic smooth muscle ELISA Kit; Actin; aortic smooth muscle ELISA Kit; ACTSA ELISA Kit; ACTVS ELISA Kit; Alpha 2 actin ELISA Kit; Alpha actin 2 ELISA Kit; Alpha cardiac actin ELISA Kit; alpha sma ELISA Kit; Alpha-actin-2 ELISA Kit; Cell growth inhibiting gene 46 protein ELISA Kit; Cell growth-inhibiting gene 46 protein ELISA Kit; GIG46 ELISA Kit; Growth inhibiting gene 46 ELISA Kit; MYMY5 ELISA Kit
缩写：ACTA2
Uniprot No.：P62736
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 91-110 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of human α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.3382.1932.2662.0731001.9311.8931.9121.719501.5471.4641.5061.313251.1121.0691.0910.89812.50.8670.8170.8420.6496.250.5240.5010.5130.3203.120.2880.2710.2800.08700.1970.1880.193 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human α-SMA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled α-SMA antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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