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产品描述：人N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AcSDKP含量。AcSDKP 是一个重要靶点。它在人体生理活动中有着一定作用，其背景涉及在细胞调节等方面的关联。研究机制聚焦于它与相关生理过程的相互作用，如对细胞增殖、分化的影响，在医学研究中有望为疾病治疗尤其是一些细胞异常疾病提供新方向。试剂盒检测范围为0.235 ng/mL-15 ng/mL，该产品可广泛应用于基础研究领域，如体外探究AcSDKP在纤维化疾病模型中的动态表达、评估药物干预对肽段水平的影响，或结合动物实验分析其在组织修复、干细胞调控等生理过程中的作用机制，为相关科研课题提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AcSDKP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.235 ng/mL-15 ng/mL
灵敏度：0.058 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AcSDKP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of human AcSDKP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 98-108 | ||||
| EDTA plasma (n=4) | 89 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected152.4652.3462.4062.2467.52.1392.0552.0971.9373.751.8121.7461.7791.6191.881.4371.5241.4811.3210.940.9620.9710.9670.8070.470.5540.5660.5600.4000.2350.3160.2970.3070.14700.1580.1610.160 | ||||||
货期：3-5 working days
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