首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β-肌动蛋白酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ACTB含量。ACTB即β-肌动蛋白，是一种高度保守的细胞骨架蛋白，广泛存在于真核细胞中。它参与维持细胞形态、细胞运动和细胞内物质运输等多种生理过程。在研究机制方面，常作为内参基因用于分析基因表达水平，以确保实验结果的准确性和可靠性。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本产品适用于细胞生物学研究、信号通路机制探索或药物干预实验中细胞活性评估，可为蛋白质表达量分析、样本均一性验证提供可靠支持，适用于实验室常规批量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A26C1A ELISA Kit; A26C1B ELISA Kit; ACTB ELISA Kit; ACTB_HUMAN ELISA Kit; Actin beta ELISA Kit; Actin cytoplasmic 1 ELISA Kit; Actin; cytoplasmic 1; N-terminally processed ELISA Kit; Actx ELISA Kit; b actin ELISA Kit; b-actin ELISA Kit; Beta cytoskeletal actin ELISA Kit; Beta-actin ELISA Kit; BRWS1 ELISA Kit; E430023M04Rik ELISA Kit; Melanoma X actin ELISA Kit; MGC128179 ELISA Kit; PS1TP5 binding protein 1 ELISA Kit; PS1TP5BP1 ELISA Kit
缩写：ACTB
Uniprot No.：P60709
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 80-105 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-100 | |||||
回收率：
| The recovery of human β-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 82-100 | ||||
| EDTA plasma (n=4) | 95 | 89-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6522.6412.6472.497102.1341.9932.0641.91451.5481.4861.5171.3672.50.9870.9600.9740.8241.250.5950.5780.5870.4370.6250.4020.3970.4000.2500.3120.2630.2450.2540.10400.1540.1450.150 | ||||||
货期：3-5 working days
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