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产品描述：人肌动蛋白 γ1 (ACTG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ACTG1含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，为细胞生物学研究、疾病模型构建及生物标志物筛选提供可靠工具；适用于体外培养细胞功能研究、组织特异性表达分析及蛋白互作机制探索等科研场景，助力研究者深入解析细胞骨架相关调控机制及其在病理生理过程中的作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACT ELISA Kit; ACTB ELISA Kit; ACTG ELISA Kit; ACTG_HUMAN ELISA Kit; actg1 ELISA Kit; Actin ELISA Kit; Actin; cytoplasmic 2 ELISA Kit; Actin; gamma 1 ELISA Kit; Actin; gamma 1 propeptide ELISA Kit; Actin; gamma ELISA Kit; BRWS2 ELISA Kit; cytoplasmic 2 ELISA Kit; Cytoskeletal gamma actin ELISA Kit; Deafness; autosomal dominant 20 ELISA Kit; Deafness; autosomal dominant 26 ELISA Kit; DFNA20 ELISA Kit; DFNA26 ELISA Kit; epididymis luminal protein 176 ELISA Kit; Gamma-actin ELISA Kit; HEL-176 ELISA Kit; N-terminally processed ELISA Kit
缩写：ACTG1
Uniprot No.：P63261
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ACTG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 101-108 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human ACTG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-98 | ||||
| EDTA plasma (n=4) | 86 | 80-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 16002.1432.2842.2142.074 8001.4411.5221.4821.342 4000.8320.8070.8200.680 2000.4930.5060.5000.360 1000.3010.3170.3090.169 500.2370.2450.2410.101 250.1890.1830.1860.046 00.1390.1400.140 | ||||||
货期：3-5 working days
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