首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠α平滑肌肌动蛋白(α-SMA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACTA2含量。ACTA2 编码α-平滑肌肌动蛋白，在血管平滑肌细胞等表达。其背景上与血管发育、收缩等生理过程相关。研究机制方面，异常表达与血管疾病、肿瘤进展等密切相关，如在血管重塑中起作用，影响肿瘤微环境和转移，是研究疾病发病机理与治疗靶点的方向。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于心血管疾病模型研究、肝肺纤维化机制探索、干细胞分化鉴定及创伤愈合动态监测等科研领域，支持体外样本中α - SMA的精准定量分析，为探索平滑肌相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acta2 ELISA kit; Actsa ELISA kit; ActvsActin ELISA kit; aortic smooth muscle ELISA kit; Alpha-actin-2) [Cleaved into: Actin ELISA kit; aortic smooth muscle ELISA kit; intermediate form] ELISA kit
缩写：ACTA2
Uniprot No.：P62738
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 97-109 | |||||
回收率：
| The recovery of rat α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 93 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.5952.4512.5232.431251.6891.6011.6451.55312.50.9720.8930.9330.8416.250.4930.5120.5030.4113.130.3300.3060.3180.2261.560.2060.2100.2080.1160.780.1260.1210.1240.03200.0940.0890.092 | ||||||
货期：3-5 working days
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