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产品描述：人抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACP5含量。ACP5即抗酒石酸酸性磷酸酶，是一种存在于破骨细胞等中的酸性磷酸酶。它在骨代谢中发挥重要作用，参与破骨细胞对骨组织的吸收过程。研究多聚焦其在骨相关疾病如骨质疏松等发病机制中的作用，通过调节其活性有望为相关疾病治疗提供新思路。试剂盒检测范围为0.312 mIU/mL-20 mIU/mL，适用于体外实验环境下的基础科研及生物医学研究，例如骨组织病理学分析、药物干预效果评估或疾病生物标志物探索等场景；为骨代谢机制研究、骨质疏松药物开发及骨骼相关疾病模型构建提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：phosphatase ELISA Kit; Acid phosphatase 5 tartrate resistant ELISA Kit; Acid phosphatase 5; tartrate resistant ELISA Kit; ACP5 ELISA Kit; EC 3.1.3.2 ELISA Kit; MGC117378 ELISA Kit; PPA5_HUMAN ELISA Kit; serum band 5 tartrate-resistant acid phosphatase ELISA Kit; SPENCDI ELISA Kit; T5ap ELISA Kit; Tartrate resistant acid ATPase ELISA Kit; Tartrate resistant acid phosphatase type 5 ELISA Kit; Tartrate resistant acid phosphatase type 5 precursor ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR AP ELISA Kit; TR-AP ELISA Kit; TRACP 5 ELISA Kit; TRAP ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
缩写：ACP5
Uniprot No.：P13686
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 mIU/mL-20 mIU/mL
灵敏度：0.078 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 100-108 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-97 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 202.4842.4562.4702.357 101.9181.8761.8971.784 51.2621.2511.2571.144 2.50.6660.7150.6910.578 1.250.4240.4540.4390.326 0.6250.3120.3290.3210.208 0.3120.1990.2080.2040.091 00.1110.1150.113 | ||||||
货期：3-5 working days
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