首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠乙酰胆碱酯酶(AChE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACHE含量。乙酰胆碱酯酶（ACHE）是神经系统中一种关键酶，主要存在于胆碱能神经元、神经肌肉接头处等，负责降解乙酰胆碱，终止神经递质传递。其研究机制涉及抑制ACHE活性以治疗神经退行性疾病，如阿尔茨海默病。通过抑制ACHE，可以增加乙酰胆碱水平，改善神经信号传递，从而缓解疾病症状。试剂盒检测范围为7.8 pg/mL-500 pg/mL，支持神经科学、毒理学及药物开发等领域研究，例如评估环境毒素对胆碱能系统的影响、筛选神经保护化合物或探究神经系统疾病的分子机制。为探索AChE在生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AcheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
缩写：ACHE
Uniprot No.：P37136
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:400 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:800 | Average % | 99 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of rat AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.3612.3842.3732.2322501.6361.6581.6471.5061251.0441.0571.0510.91062.50.6100.5990.6050.46431.20.4130.4040.4090.26815.60.3050.2970.3010.1607.80.2370.2280.2330.09200.1420.1400.141 | ||||||
货期：3-5 working days
×
