首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠乙酰胆碱酯酶(AChE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的AChE含量。AChE即乙酰胆碱酯酶，是胆碱能神经系统中的关键酶，可催化乙酰胆碱水解。其背景在于对神经系统功能维持至关重要。研究机制主要围绕其结构、活性调节及与底物作用等方面，旨在了解其在生理病理过程作用，为相关疾病治疗提供靶点。试剂盒检测范围为0.312 mU/mL-20 mU/mL，适用于科研实验中评估神经功能调控、药物毒性测试或疾病模型（如阿尔茨海默症、有机磷中毒）的AChE动态变化；为神经生物学、药理学及毒理学研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ache ELISA Kit; Acetylcholinesterase ELISA Kit; AChE ELISA Kit; EC 3.1.1.7 ELISA Kit
缩写：AChE
Uniprot No.：P21836
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 mU/mL-20 mU/mL
灵敏度：0.078 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 91-104 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 88-102 | |||||
回收率：
| The recovery of mouse AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-101 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected202.3132.4132.3632.260101.4981.5981.5481.44550.8400.8300.8350.7322.50.4790.4890.4840.3811.250.3060.3160.3110.2080.6250.1970.2070.2020.0990.3120.1350.1460.1410.03800.1030.1030.103 | ||||||
本试剂盒所含材料：
显示更多
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse AChE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled AChE antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
