首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠8羟基脱氧鸟苷(8-OHdG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的8-OHdG含量。8-OHdG（8-羟基脱氧鸟苷）是DNA中的一种氧化损伤产物，由活性氧自由基攻击DNA分子中的鸟嘌呤碱基第8位碳原子产生。它是氧化应激和DNA损伤的重要生物标志物，与多种疾病如癌症、心血管疾病和神经退行性疾病有关。研究8-OHdG有助于了解DNA损伤和修复过程，以及评估疾病风险。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外细胞模型、动物实验中的氧化应激评估，如药物抗氧化效果验证、环境污染因子（如重金属、辐射）的DNA损伤研究，以及脑组织、肝脏等器官的氧化损伤机制分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：8-OHdG
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 98-107 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 201.8901.7801.8351.682 101.1981.1281.1631.010 50.7560.7260.7410.588 2.50.5250.4950.5100.357 1.250.3210.3180.3200.167 0.6250.2720.2620.2670.114 0.3120.2130.2090.2110.058 00.1540.1520.153 | ||||||
货期：3-5 working days
×
