首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠8羟基脱氧鸟苷(8-OHdG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的8-OHdG含量。8-OHdG 是 DNA 氧化损伤的生物标志物。其背景在于机体受内外界因素影响产生氧化应激，导致 DNA 中鸟嘌呤氧化形成 8-OHdG。研究机制多围绕检测其含量来评估氧化应激水平、DNA 损伤程度，及与肿瘤、神经退行性疾病等的关联。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于神经退行性疾病模型、衰老机制研究、环境毒理学评估及代谢性疾病等科研领域，可为氧化损伤相关机制研究、药物筛选及抗氧化剂功效评价提供数据支持，广泛应用于基础医学、药理学及生物化学等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：8-OHdG
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 87-97 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 93-104 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 82-91 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 92 | 88-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 500.0710.0740.073 250.1200.1300.125 12.50.2000.2060.203 6.250.3350.3400.338 3.120.5730.5540.564 1.560.8580.8410.850 0.781.1291.1391.134 02.2152.1852.200 | ||||||
货期：3-5 working days
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