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产品描述：牛α2巨球蛋白(A2M)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的A2M含量。A2M即α₂-巨球蛋白，是血浆中一种多功能糖蛋白。它能抑制多种蛋白水解酶活性，参与细胞的生长、修复等过程。在研究机制上，主要关注其对蛋白酶的捕获作用、与细胞表面受体的相互作用，以及在疾病发生发展中所扮演的角色。试剂盒检测范围为0.312 μg/mL- 20 μg/mL，适用于科研场景中牛类动物模型的炎症机制研究、免疫调控分析或蛋白酶活性相关疾病模型的分子机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A2MAlpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
缩写：A2M
Uniprot No.：Q7SIH1
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 μg/mL- 20 μg/mL
灵敏度：0.078 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine A2M in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 98 | ||||
| Range % | 90-103 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 82-94 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 95-106 | |||||
| 1:800 | Average % | 108 | ||||
| Range % | 101-112 | |||||
回收率：
| The recovery of bovine A2M spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-91 | ||||
| EDTA plasma (n=4) | 93 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average200.1270.1310.129100.2480.2330.24150.4900.5230.5072.50.9090.9110.9101.251.4391.4641.4520.6251.8531.7891.8210.3122.4602.4002.43002.7952.7022.749 | ||||||
货期：3-5 working days
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