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产品描述：人8异前列腺素F2α（8-iso-PGF2a）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的8-iso-PGF2a含量。试剂盒检测范围为125 pg/mL-5000 pg/mL，适用于细胞氧化损伤机制研究、抗氧化药物筛选评价以及环境或营养因素对机体氧化应激影响的科学探索，为氧化应激相关领域的基础研究与转化医学提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：8-iso prostaglandin F2α,8-iso prostaglandin F2 alpha,8-iso-PGF2α,8-iso-PGF2 alpha
缩写：8-iso-PGF2a
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：125 pg/mL-5000 pg/mL
灵敏度：125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 8-iso-PGF2a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of human 8-iso-PGF2a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 88-102 | ||||
| EDTA plasma (n=4) | 97 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 50000.1240.1260.125 25000.2190.2320.226 11500.3410.3540.348 3750.6050.6460.626 1251.2731.3051.289 02.6542.5512.603 | ||||||
货期：3-5 working days
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