Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<12%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E10140h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人8羟基脱氧鸟苷(8-OHdG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、尿液样本中的8-OHdG含量。8-OHdG是细胞内DNA氧化应激损伤的标志性产物。其背景在于机体受内外部因素影响产生氧化应激，损伤DNA生成8-OHdG。研究机制方面，常检测其水平以评估氧化应激程度，在疾病早期诊断、病情监测及药物研发等领域有重要意义。试剂盒检测范围为 3.12 ng/mL-800 ng/mL，该产品适用于体外定量评估生物样本中的氧化损伤水平，为探索氧化应激与疾病关联机制、药物筛选及毒理学研究提供高效工具，尤其适合需要高灵敏度和宽线性范围的科研场景，满足血清和尿液等易获取样本的便捷检测需求。在衰老、肿瘤发生、神经退行性疾病及环境毒理等研究领域具有重要科研价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
8-OHdG
种属：
Homo sapiens (Human)
样本类型：
serum, urine
检测范围：
3.12 ng/mL-800 ng/mL
灵敏度：
3.12 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-91
1:2	Average %	87
	Range %	81-95
1:4	Average %	88
	Range %	82-95
1:8	Average %	86
	Range %	81-92

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
800	0.337	0.344	0.340	0.340
200	0.873	0.857	0.865	0.865
50	1.302	1.328	1.315	1.315
12.5	1.784	1.699	1.741	1.741
3.12	2.190	2.291	2.240	2.240
0	2.534	2.453	2.493	2.493