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产品描述：人8-异构前列腺素F2α(8-epi-PGF2α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的8-epi-PGF2α含量。试剂盒检测范围为78 pg/mL-5000 pg/mL，该产品适用于体外研究样本中氧化应激水平的动态监测，可应用于动物模型或细胞实验中评估抗氧化治疗效果、疾病进程中氧化损伤程度等科研场景，为探索氧化应激相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：8-epi-prostaglandin F2α,8-epi-PGF2α,8-epi-PGF2 alpha
缩写：8-epi-PGF2α
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 8-epi-PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 87-103 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-103 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-100 | |||||
回收率：
| The recovery of human 8-epi-PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.9652.8972.9312.82025002.2112.3232.2672.15612501.5991.5661.5831.4726250.8110.8020.8070.6963120.5050.4740.4900.3791560.3110.3010.3060.195780.2510.2340.2430.13200.1130.1090.111 | ||||||
货期：3-5 working days
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