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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||||||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||||||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||||||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||||||||||
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)5.8286.0526.2505.8285.9706.314SD0.0340.0390.0450.0380.0490.055CV(%)4.0434.5195.1024.5185.7316.194 |
To assess the linearity of the assay, samples were spiked with high concentrations of human Trypsin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||||||||
Sample | Serum(n=4) | |||||||||||
1:1 | Average % | 92 | ||||||||||
Range % | 87-98 | |||||||||||
1:2 | Average % | 98 | ||||||||||
Range % | 93-104 | |||||||||||
1:4 | Average % | 84 | ||||||||||
Range % | 82-88 | |||||||||||
1:8 | Average % | 90 | ||||||||||
Range % | 85-97 |
The recovery of human Trypsin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||||||||||
Sample Type | Average % Recovery | Range | ||||||||||||
Serum (n=5) | 95 | 89-99 | ||||||||||||
EDTA plasma (n=4) | 96 | 90-102 | ||||||||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||
ng/ml | OD1 | OD2 | Average | Corrected | |||||||||||
50 | 2.432 | 2.389 | 2.411 | 2.265 | |||||||||||
25 | 1.822 | 1.831 | 1.827 | 1.681 | |||||||||||
12.5 | 1.276 | 1.281 | 1.279 | 1.133 | |||||||||||
6.25 | 0.819 | 0.804 | 0.812 | 0.666 | |||||||||||
3.12 | 0.431 | 0.434 | 0.433 | 0.287 | |||||||||||
1.56 | 0.234 | 0.243 | 0.239 | 0.093 | |||||||||||
0.78 | 0.189 | 0.184 | 0.187 | 0.041 | |||||||||||
0 | 0.149 | 0.142 | 0.146 | ||||||||||||
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