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Human Trypsin-2(PRSS2) ELISA kit

  • 中文名: 人胰蛋白酶2(PRSS2)ELISA kit
货号: IPD-E09623h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人胰蛋白酶原Ⅱ(Try-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的Try含量。试剂盒检测范围为0.78 ng/ml-50 ng/ml,灵敏度为0.19 ng/ml。为研究胰腺外分泌功能、探索相关疾病分子机制、评估实验性治疗干预效果等科研领域提供可靠工具,适用于基础医学研究、生物标志物筛选及药物开发等非诊断性科研场景。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Anionic trypsinogen ELISA Kit; EC 3.4.21.4 ELISA Kit; MGC111183 ELISA Kit; MGC120174 ELISA Kit; Protease serine 2 preproprotein ELISA Kit; Protease; serine; 2 (trypsin 2) ELISA Kit; Protease; serine; 2 ELISA Kit; Protease; serine; 2; preproprotein ELISA Kit; Prss2 ELISA Kit; Serine protease 2 ELISA Kit; TRY2 ELISA Kit; TRY2_HUMAN ELISA Kit; TRY8 ELISA Kit; TRYP2 ELISA Kit; Trypsin 2 ELISA Kit; Trypsin II ELISA Kit; Trypsin-2 ELISA Kit; Trypsinogen 2 ELISA Kit
  • 缩写:
    Try
  • Uniprot No.:
    P07478
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.78 ng/ml-50 ng/ml.
  • 灵敏度:
    0.39 ng/m
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.




    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.





     Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)5.8286.0526.2505.8285.9706.314SD0.0340.0390.0450.0380.0490.055CV(%)4.0434.5195.1024.5185.7316.194

  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human Trypsin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)




    1:1

    Average %

    92




    Range %

    87-98




    1:2

    Average %

    98




    Range %

    93-104




    1:4

    Average %

    84




    Range %

    82-88




    1:8

    Average %

    90




    Range %

    85-97




  • 回收率:

    The recovery of human Trypsin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.





    Sample Type

    Average % Recovery

    Range






    Serum (n=5)

    95

    89-99






    EDTA plasma (n=4)

    96

    90-102













  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



















    ng/ml

    OD1

    OD2

    Average

    Corrected





    50

    2.432

    2.389

    2.411

    2.265





    25

    1.822

    1.831

    1.827

    1.681





    12.5

    1.276

    1.281

    1.279

    1.133





    6.25

    0.819

    0.804

    0.812

    0.666





    3.12

    0.431

    0.434

    0.433

    0.287





    1.56

    0.234

    0.243

    0.239

    0.093





    0.78

    0.189

    0.184

    0.187

    0.041





    0

    0.149

    0.142

    0.146















  • 货期:
    3-5 working days

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