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产品描述:人分泌性白细胞蛋白酶抑制因子(SLPI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SLPI含量。SLPI(分泌性白细胞蛋白酶抑制剂)是一种酸稳定的蛋白酶抑制剂,具有抗炎和免疫调节功能。它在细菌感染和单核细胞增生李斯特菌感染后的炎症和免疫反应中发挥关键作用,同时参与调节NF-κB的激活,影响炎症反应。研究显示,SLPI在肿瘤生长与侵袭中发挥作用,其表达模式与癌症和呼吸系统疾病等多种疾病有关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,灵敏度为7.81 pg/mL。为研究感染性疾病进程、炎症微环境调控或黏膜屏障功能等课题提供可靠工具;支持科研人员在体外实验中系统性探索SLPI在免疫应答、病原体防御及再生医学等领域的生物学功能。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:ALK 1 ELISA Kit; ALK1 ELISA Kit; ALP ELISA Kit; Antileukoprotease ELISA Kit; Antileukoproteinase 1 ELISA Kit; Antileukoproteinase ELISA Kit; Antileukoproteinase1 ELISA Kit; BLPI ELISA Kit; Human seminal protease inhibitor ELISA Kit; HUSI 1 ELISA Kit; HUSI ELISA Kit; HUSI I ELISA Kit; HUSI-1 ELISA Kit; MPI ELISA Kit; Mucus proteinase inhibitor ELISA Kit; Protease inhibitor WAP4 ELISA Kit; Secretory leukocyte peptidase inhibitor ELISA Kit; Secretory leukocyte protease inhibitor ELISA Kit; Seminal proteinase inhibitor ELISA Kit; SLPI ELISA Kit; SLPI_HUMAN ELISA Kit; WAP four disulfide core domain protein 4 ELISA Kit; WAP four-disulfide core domain protein 4 ELISA Kit; WAP4 ELISA Kit; WFDC4 ELISA Kit
缩写:SLPI
Uniprot No.:P03973
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.81 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SLPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:100 | Average % | 96 | |||||
Range % | 91-102 | ||||||
1:200 | Average % | 92 | |||||
Range % | 85-98 | ||||||
1:400 | Average % | 89 | |||||
Range % | 83-94 | ||||||
1:800 | Average % | 91 | |||||
Range % | 87-96 |
回收率:
The recovery of human SLPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 97 | 93-102 | |||||
EDTA plasma (n=4) | 86 | 81-92 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected 20002.6372.5312.5842.490 10002.1242.2052.1652.071 5001.4541.5571.5061.412 2500.9360.9230.9300.836 1250.4480.4780.4630.369 62.50.2830.2740.2790.185 31.250.1560.1630.1600.066 00.0930.0950.094 |
货期:3-5 working days
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