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Human anti-Mullerian hormone (AMH) ELISA kit

  • 中文名： 人缪勒管抑制物质/抗缪勒管激素(AMH)ELISA kit
货号: IPD-E12756h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人缪勒管抑制物质/抗缪勒管激素(AMH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AMH含量。抗缪勒管激素（AMH）在生殖领域背景重要，它由卵巢颗粒细胞分泌，可反映卵巢储备功能。研究机制上，AMH通过与特定受体结合，参与卵泡发育的调节，抑制原始卵泡募集及卵泡生长，为评估女性生育力等研究提供关键依据。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。适用于生殖生物学研究、内分泌调控机制探索以及疾病模型中激素水平变化的评估。科研人员可通过分析卵巢储备功能、睾丸支持细胞活性或相关动物模型的性腺发育状态，为生殖系统疾病机制研究、药物干预效果评价等提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AMH ELISA Kit; MIF ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
  • 缩写：
    AMH
  • Uniprot No.：
    P03971
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)492.045500.047507.604498.579504.986493.245SD0.0210.0350.0300.0450.0430.039CV(%)3.4775.7164.7927.2936.9026.373
  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    85-96


    1:2

    Average %

    96


    Range %

    90-108


    1:4

    Average %

    98


    Range %

    95-100


    1:8

    Average %

    97


    Range %

    90-105









  • 回收率：

    The recovery of human AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-101


    EDTA plasma (n=4)

    97

    90-105









  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected40002.6562.7642.7102.58220001.7101.8731.7921.66410001.0961.0621.0790.9515000.5990.6090.6040.4762500.3730.3530.3630.2351250.2680.2640.2660.13862.50.2040.2210.2130.08500.1250.1310.128







  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AMH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled AMH antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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