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产品描述：人基质金属蛋白酶9/明胶酶B(MMP-9/Gelatinase B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液、尿液样本中的MMP9含量。MMP9，即基质金属蛋白酶-9，是MMP家族的成员，参与细胞外基质分解，在胚胎发育、生殖、伤口修复等生理过程中发挥作用。MMP9在多种疾病中起作用，如关节炎、动脉粥样硬化、肿瘤侵袭等，其研究机制涉及蛋白裂解、细胞外基质降解等过程。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.284 ng/mL。支持科研人员开展肿瘤微环境分析、炎症反应研究、组织修复机制探索及泌尿系统相关疾病模型构建等领域的定量检测需求，为探索MMP - 9在疾病发生发展中的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：82 kDa matrix metalloproteinase-9 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; CLG 4B ELISA Kit; CLG4B ELISA Kit; Collagenase Type 4 beta ELISA Kit; Collagenase type IV 92 KD ELISA Kit; EC 3.4.24.35 ELISA Kit; Gelatinase 92 KD ELISA Kit; Gelatinase B ELISA Kit; Gelatinase beta ELISA Kit; GelatinaseB ELISA Kit; GELB ELISA Kit; Macrophage gelatinase ELISA Kit; MANDP2 ELISA Kit; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) ELISA Kit; Matrix Metalloproteinase 9 ELISA Kit; MMP 9 ELISA Kit; MMP-9 ELISA Kit; MMP9 ELISA Kit; MMP9_HUMAN ELISA Kit; Type V collagenase ELISA Kit
缩写：MMP9
Uniprot No.：P14780
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates, urine
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.284 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MMP-9 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled MMP-9 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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