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Human Macrophage Inflammatory Protein-3β,MIP-3β ELISA kit

  • 中文名： 人巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA Kit
货号: IPD-E04670h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL19含量。CCL19是重要的靶点。它是趋化因子，参与淋巴细胞迁移与归巢。在免疫调节、炎症反应等方面有重要作用。目前研究聚焦于其在自身免疫病、肿瘤等疾病中的机制，期望通过干预该靶点治疗相关疾病。试剂盒检测范围为39 pg/ml-2500 pg/ml，灵敏度为9.75 pg/ml。适用于免疫学基础研究、炎症性疾病模型构建、药物靶点筛选等方向，例如在探索类风湿性关节炎、炎症性肠病等发病机制时，可通过检测CCL19表达水平变化评估免疫细胞募集状态；支持科研人员高效开展体外样本中趋化因子动态变化的定量研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Beta chemokine exodus 3 ELISA Kit; Beta-chemokine exodus-3 ELISA Kit; C C chemokine ligand 19 ELISA Kit; C-C motif chemokine 19 ELISA Kit; CC chemokine ligand 19 ELISA Kit; CCL 19 ELISA Kit; CCL19 ELISA Kit; CCL19_HUMAN ELISA Kit; Chemokine (C C motif) ligand 19 ELISA Kit; Chemokine (CC motif) ligand 19 ELISA Kit; Chemokine C C Motif Ligand 19 ELISA Kit; Chemokine CC Motif Ligand 19 ELISA Kit; CK beta 11 ELISA Kit; CK beta-11 ELISA Kit; CKb 11 ELISA Kit; CKb11 ELISA Kit; EBI 1 ligand chemokine ELISA Kit; EBI1 ligand chemokine ELISA Kit; ELC ELISA Kit; Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit; Exodus 3 ELISA Kit; Exodus3 ELISA Kit; Macrophage inflammatory protein 3 beta ELISA Kit; MGC34433 ELISA Kit; MIP 3 beta ELISA Kit; MIP 3B ELISA Kit; MIP-3-beta ELISA Kit; MIP-3b ELISA Kit; MIP3 beta ELISA Kit; MIP3B ELISA Kit; OTTHUMP00000000531 ELISA Kit; SCYA 19 ELISA Kit; SCYA19 ELISA Kit; Small inducible cytokine A19 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 1 ELISA Kit; Small-inducible cytokine A19 ELISA Kit
  • 缩写：
    CCL19
  • Uniprot No.：
    Q99731
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 pg/ml-2500 pg/ml
  • 灵敏度：
    9.75 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)320.879331.93326.404321.8324.562324.122SD0.0410.0400.0380.0520.0460.055CV(%)5.2975.0134.8356.7005.8827.039







  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-3β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)

    1:1

    Average %

    83

    Range %

    80-85

    1:2

    Average %

    92

    Range %

    87-96

    1:4

    Average %

    101

    Range %

    97-104

    1:8

    Average %

    87

    Range %

    85-89








  • 回收率：

    The recovery of human MIP-3β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    87-93

    EDTA plasma (n=4)

    104

    101-108








  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2500

    2.574

    2.638

    2.606

    2.479

    1250

    2.009

    2.001

    2.005

    1.898

    625

    1.309

    1.402

    1.356

    1.229

    312.5

    0.774

    0.798

    0.786

    0.659

    156.25

    0.401

    0.398

    0.400

    0.273

    78

    0.264

    0.268

    0.266

    0.139

    39

    0.193

    0.187

    0.190

    0.063

    0

    0.126

    0.128

    0.127










  • 货期：
    3-5 working days

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