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Human pulmonary activation regulated chemokine,PARC ELISA Kit

  • 中文名： 人肺部活化调节趋化因子(PARC/CCL18)ELISA Kit
货号: IPD-E09941h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人肺部活化调节趋化因子(PARC/CCL18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CCL18含量。CCL18是一种重要靶点。它属于趋化因子家族，在免疫调节等生理过程中发挥作用。研究发现其在肿瘤微环境、免疫性疾病中表达异常。机制上，它能招募免疫细胞、影响细胞迁移和活化，为相关疾病的治疗和研究提供了方向。试剂盒检测范围为31.25 pg/ml-2000 pg/ml，灵敏度为7.81 pg/ml。适用于体外实验中探究肺部疾病模型、免疫调控机制、药物干预效果等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Alternative macrophage activation associated CC chemokine 1 ELISA Kit; Alternative macrophage activation-associated CC chemokine 1 ELISA Kit; AMAC-1 ELISA Kit; AMAC1 ELISA Kit; CC chemokine PARC ELISA Kit; CCL18 ELISA Kit; CCL18(4-69) ELISA Kit; CCL18_HUMAN ELISA Kit; CKb7 ELISA Kit; DC-CK1 ELISA Kit; DCCK1 ELISA Kit; DCCK1 ELISA Kit; Dendritic cell chemokine 1 ELISA Kit; Macrophage inflammatory protein 4 ELISA Kit; MIP-4 ELISA Kit; MIP4 ELISA Kit; PARC ELISA Kit; Pulmonary and activation regulated chemokine ELISA Kit; Pulmonary and activation-regulated chemokine ELISA Kit; SCYA18 ELISA Kit; Small inducible cytokine A18 ELISA Kit; Small inducible cytokine A18 ELISA Kit; Small-inducible cytokine A18 ELISA Kit
  • 缩写：
    CCL18
  • Uniprot No.：
    P55774
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/ml-2000 pg/ml
  • 灵敏度：
    7.81 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.



    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.








    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)244.406249.006237.517236.211239.732235.807SD0.0450.0550.0520.0660.0720.078CV(%)4.0114.6854.5105.8206.0816.915
  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human PARC/CCL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.




    Sample

    Serum(n=4)



    1:100

    Average %

    91



    Range %

    87-94



    1:200

    Average %

    97



    Range %

    95-103



    1:400

    Average %

    98



    Range %

    95-102



    1:800

    Average %

    93



    Range %

    91-98










  • 回收率：

    The recovery of human PARC/CCL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.



    Sample Type

    Average % Recovery

    Range



    Serum (n=5)

    91

    87-94



    EDTA plasma (n=4)

    98

    95-102










  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


























































    pg/ml

    OD1

    OD2

    Average

    Corrected



    2000

    2.466

    2.585

    2.526

    2.394



    1000

    1.991

    1.944

    1.968

    1.836



    500

    1.482

    1.567

    1.525

    1.393



    250

    1.213

    1.130

    1.172

    1.040



    125

    0.832

    0.900

    0.866

    0.734



    62.5

    0.477

    0.450

    0.464

    0.332



    31.25

    0.244

    0.251

    0.248

    0.116



    0

    0.129

    0.135

    0.132





  • 货期：
    3-5 working days

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