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产品描述：人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFR-3/Flt-4含量。VEGFR-3/Flt-4 是血管内皮生长因子受体，在淋巴管生成和血管生成中起关键作用。其通过与配体结合激活下游信号通路，调节内皮细胞的增殖、迁移等，目前在肿瘤血管生成、淋巴管转移等研究中受关注，为抗肿瘤药物研发提供了靶点思路。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。适用于体外实验、疾病模型研究及生物标志物探索等科研领域，例如肿瘤微环境中淋巴管生成的机制解析、心血管疾病相关因子调控研究，或通过检测细胞培养上清、实验动物体液及病理组织中的VEGFR - 3动态变化，为肿瘤转移、淋巴水肿等疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 2.7.10.1 ELISA Kit; flt 4 ELISA Kit; FLT-4 ELISA Kit; FLT4 ELISA Kit; FLT41 ELISA Kit; Fms related tyrosine kinase 4 ELISA Kit; Fms-like tyrosine kinase 4 ELISA Kit; LMPH1A ELISA Kit; PCL ELISA Kit; Soluble VEGFR3 variant 1 ELISA Kit; Soluble VEGFR3 variant 2 ELISA Kit; Soluble VEGFR3 variant 3 ELISA Kit; Tyrosine protein kinase receptor FLT4 ELISA Kit; Tyrosine-protein kinase receptor FLT4 ELISA Kit; Vascular endothelial growth factor receptor 3 ELISA Kit; Vascular endothelial growth factor receptor 3 precursor ELISA Kit; VEGF R3 ELISA Kit; VEGFR 3 ELISA Kit; VEGFR-3 ELISA Kit; VEGFR3 ELISA Kit; VGFR3_HUMAN ELISA Kit
缩写：FLT4
Uniprot No.：P35916
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-3/Flt-44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 91-105 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 85-96 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 85-105 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 83-98 | ||||||
回收率：
| The recovery of human VEGFR-3/Flt-44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 86-100 | |||||
| EDTA plasma (n=4) | 93 | 87-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.5492.5162.5332.420 51.5171.5571.5371.424 2.50.7960.7580.7770.664 1.250.4720.4890.4810.368 0.6250.3020.3150.3090.196 0.3120.2590.2460.2530.140 0.1560.1640.1710.1680.055 00.1110.1150.113 | |||||||
货期：3-5 working days
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