Human Vascular Endothelial cell Growth Factor D,VEGF-D ELISA KIT

中文名： 人血管内皮细胞生长因子D(VEGF-D)ELISA Kit

货号: IPD-E04760h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人血管内皮细胞生长因子D(VEGF-D)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的VEGFD含量。VEGFD是重要的靶点。它属于血管内皮生长因子家族，在淋巴管生成和血管生成中发挥关键作用。研究机制主要聚焦于其与受体结合激活下游信号通路，影响细胞增殖、迁移等。对VEGFD的研究有望为肿瘤及淋巴管相关疾病治疗带来新策略。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml，灵敏度为7.81 pg/ml。适用于科研领域中对VEGF - D表达动态的定量分析，如肿瘤生物学研究中转移机制探索、心血管疾病模型中的血管生成评估，或炎症相关疾病动物模型的组织病理学研究，为体外实验、药物干预效果评价及分子机制解析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
c-fos induced growth factor ELISA Kit; c-fos induced growth factor (vascular endothelial growth factor D) ELISA Kit; c-fos induced growth factor ELISA Kit; c-fos-induced growth factor ELISA Kit; FIGF ELISA Kit; Vascular endothelial growth factor D ELISA Kit; Vascular endothelial growth factor D precursor ELISA Kit; Vascular endothelial growth factor D precursor ELISA Kit; VEGF D ELISA Kit; VEGF-D ELISA Kit; VEGFD ELISA Kit; VEGFD_HUMAN ELISA Kit
缩写：
FIGF
Uniprot No.：
O43915
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/ml - 2000 pg/ml
灵敏度：
7.81 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)250.031251.057246.271248.467251.258249.008SD0.0340.0290.0380.0510.0540.055CV(%)4.1163.4984.6636.2096.5096.683

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

88-98

1:2

Average %

102

Range %

92-106

1:4

Average %

Range %

89-102

1:8

Average %

Range %

87-98

回收率：

The recovery of human VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	91	84-96
EDTA plasma (n=4)	99	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












pg/ml	OD1	OD2	Average	Corrected
2000	2.436	2.568	2.502	2.352
1000	2.062	2.132	2.097	1.947
500	1.457	1.444	1.451	1.301
250	0.760	0.789	0.775	0.625
125	0.486	0.458	0.472	0.322
62.5	0.372	0.360	0.366	0.216
31.25	0.255	0.247	0.251	0.101
0	0.152	0.148	0.150