Mouse Monoclonal POLR2A Antibody

The POLR2A antibody targets the largest subunit of RNA polymerase II (RNAP II), a critical enzyme responsible for transcribing protein-coding genes in eukaryotic cells. Encoded by the POLR2A gene, this subunit (RPB1) forms the catalytic core of RNAP II and contains structural domains essential for transcription initiation, elongation, and termination. Notably, its C-terminal domain (CTD) features repetitive heptapeptide sequences that undergo dynamic phosphorylation during the transcription cycle, regulating interactions with transcription factors and mRNA processing machinery. POLR2A antibodies are widely used in research to study transcriptional mechanisms, RNAP II localization, and cell cycle-dependent phosphorylation states (e.g., using clones like 8WG16 or phospho-specific variants). They are applied in techniques such as Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence. Dysregulation of POLR2A is linked to cancers, neurodegenerative diseases, and developmental disorders, making its antibodies valuable tools for investigating disease-associated transcriptional dysfunctions. Additionally, these antibodies aid in exploring therapeutic agents targeting transcriptional vulnerabilities, underscoring their importance in both basic research and translational medicine.