| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 929 |
| clone | 1D1F8 |
| WB Predicted band size | 40kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD14 (AA: 230-345) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于CD14抗体的经典文献示例(仅供参考，具体文献需核实数据库)：
1. **文献名称**: *CD14. a receptor for complexes of lipopolysaccharides with lipopolysaccharide binding protein*
**作者**: Wright SD et al.
**摘要**: 该研究首次阐明CD14作为脂多糖(LPS)与脂多糖结合蛋白复合物的受体功能，揭示其在先天免疫中识别革兰氏阴性菌的关键作用。
2. **文献名称**: *MD-2 enables Toll-like receptor 4 (TLR4)-mediated macrophage activation by lipopolysaccharide*
**作者**: Gangloff SC et al.
**摘要**: 探讨CD14与TLR4/MD-2复合物的协同作用，证明CD14抗体可阻断LPS诱导的炎症信号通路，为抗炎治疗提供靶点。
3. **文献名称**: *CD14 expression and soluble levels in human subsets of blood monocytes*
**作者**: Ziegler-Heitbrock HW et al.
**摘要**: 通过CD14抗体区分单核细胞亚群，发现CD14高表达亚群在炎症反应中起主导作用，为免疫细胞分型提供标志物。
4. **文献名称**: *CD14 as a biomarker for cardiovascular diseases*
**作者**: Antal-Szalmás P et al.
**摘要**: 提出可溶性CD14(sCD14)水平与动脉粥样硬化的相关性，CD14抗体检测可用于心血管疾病风险评估。
**备注**：建议通过PubMed或Google Scholar搜索最新文献，并核对DOI获取全文。
CD14 is a glycosylphosphatidylinositol (GPI)-anchored cell surface antigen that functions as a co-receptor for lipopolysaccharide (LPS), a key component of Gram-negative bacterial membranes. Primarily expressed on monocytes, macrophages, neutrophils, and dendritic cells, it plays a critical role in innate immune responses by binding LPS and facilitating its recognition by the Toll-like receptor 4 (TLR4)/MD-2 complex. CD14 exists in both membrane-bound (mCD14) and soluble (sCD14) forms, with the latter acting as a decoy receptor to modulate inflammatory responses.
CD14 antibodies are essential tools for identifying and isolating specific immune cell subsets. They are widely used in flow cytometry to distinguish monocyte subsets (e.g., classical CD14++CD16− vs. non-classical CD14+CD16+ monocytes) and to study cellular activation states. In research, these antibodies help elucidate mechanisms of pathogen recognition, cytokine production, and sepsis pathology. Clinically, CD14 expression levels correlate with diseases like atherosclerosis, rheumatoid arthritis, and septic shock, making it a biomarker of interest. Therapeutic applications include neutralizing LPS toxicity and targeting CD14 in inflammatory disorders. Validation of CD14 antibodies requires attention to cross-reactivity and epitope specificity due to structural similarities with other LPS-binding proteins.
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