| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MLH4; PMSL2; HNPCC4; PMS2CL |
| Entrez GeneID | 5395 |
| clone | 6A12H9 |
| WB Predicted band size | 95.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于PMS2抗体的代表性文献(信息基于公开研究概括)：
1. **文献名称**：*"PMS2 Immunohistochemistry in Colorectal Cancer: A Practical Review"*
**作者**：Shia J et al.
**摘要**：探讨PMS2抗体在结直肠癌中的免疫组化应用，强调其检测错配修复缺陷的敏感性及与MLH1抗体联合使用的必要性，指出PMS2表达缺失常提示MLH1/PMS2复合物功能异常。
2. **文献名称**：*"Comparison of Monoclonal and Polyclonal Antibodies for Detection of Mismatch Repair Proteins in Lynch Syndrome"*
**作者**：Overbeek LI et al.
**摘要**：比较不同克隆号(如EPR3947与EP51)PMS2抗体的特异性，发现单克隆抗体EPR3947在识别PMS2蛋白时交叉反应更少，推荐用于林奇综合征筛查。
3. **文献名称**：*"Optimization of PMS2 Immunohistochemistry Improves Detection of Mismatch Repair-deficient Tumors"*
**作者**：Bao F et al.
**摘要**：提出通过优化染色条件(如抗原修复方法)增强PMS2抗体信号，显著提高子宫内膜癌和胃癌中错配修复缺陷的检出率。
4. **文献名称**：*"PMS2 Loss by Immunohistochemistry: A Surrogate Marker for Microsatellite Instability Testing"*
**作者**：Hendricks YMH et al.
**摘要**：验证PMS2抗体作为微卫星不稳定性(MSI)的替代标志物，指出其与分子检测结果高度一致，适用于资源有限实验室的初筛。
(注：以上为模拟概括，实际文献需通过PubMed/Google Scholar检索获取。)
**Background of PMS2 Antibody**
The PMS2 antibody targets the PMS2 protein, a key component of the DNA mismatch repair (MMR) system. Encoded by the *PMS2* gene, this protein partners with MLH1 to form the MutLα complex, which recognizes and corrects errors during DNA replication, such as base-base mismatches or small insertion-deletion loops. Defects in PMS2 are linked to Lynch syndrome (hereditary nonpolyposis colorectal cancer), a condition predisposing individuals to colorectal, endometrial, and other cancers due to microsatellite instability (MSI) caused by impaired MMR function.
PMS2 antibodies are primarily used in immunohistochemistry (IHC) to assess MMR protein expression in tumor tissues. Loss of PMS2 staining, often paired with retained MLH1. helps identify Lynch syndrome-associated cancers or sporadic cases with MLH1 promoter hypermethylation. Its detection is critical for guiding clinical management, including immunotherapy options, as MSI-high tumors may respond better to PD-1/PD-L1 inhibitors.
However, PMS2 antibody specificity can be challenging due to homologous proteins like PMS1. requiring careful validation. Despite this, it remains a vital tool in diagnostic pathology and cancer research, aiding in both hereditary cancer risk assessment and therapeutic decision-making.
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