| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CD8; p32; Leu2 |
| Entrez GeneID | 925 |
| clone | 8H8A8 |
| WB Predicted band size | 25.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD8a (AA: 22–235) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD8α抗体的3篇代表性文献(注：文献信息为虚构示例，仅供格式参考)：
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1. **文献名称**: *CD8α Antibody-Based Flow Cytometry for Cytotoxic T Cell Profiling*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种高特异性CD8α单克隆抗体，并优化了其在流式细胞术中的应用，用于准确区分外周血中的细胞毒性T细胞(CTL)亚群。实验验证了该抗体在小鼠和人源样本中的交叉反应性。
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2. **文献名称**: *Role of CD8α+ Dendritic Cells in Antigen Presentation*
**作者**: Brown L, et al.
**摘要**: 通过CD8α抗体标记和基因敲除模型，揭示了CD8α+树突状细胞在交叉呈递肿瘤抗原中的关键作用，为癌症免疫治疗提供了新靶点。
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3. **文献名称**: *CD8α Antibody-Conjugated Nanoparticles for Targeted T Cell Modulation*
**作者**: Zhang Y, et al.
**摘要**: 利用CD8α抗体修饰的纳米颗粒特异性靶向CTL，成功实现了局部免疫调节，在自身免疫疾病模型中显著降低了病理损伤。
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如需真实文献，建议在 **PubMed** 或 **Google Scholar** 检索以下关键词：
`CD8α antibody flow cytometry`、`CD8a T cell isolation`、`CD8α+ dendritic cell function`.
CD8a antibodies are essential tools in immunology research, specifically targeting the CD8α chain, a component of the CD8 co-receptor expressed on cytotoxic T lymphocytes (CTLs) and some dendritic cell subsets. The CD8 co-receptor, typically existing as a heterodimer (CD8αβ) on CTLs or a homodimer (CD8αα) on innate-like T cells, binds to MHC class I molecules, stabilizing T cell receptor (TCR)-peptide-MHC interactions and enhancing TCR signaling. CD8a antibodies enable the identification and isolation of CD8+ T cells, which play critical roles in adaptive immunity, including antiviral and antitumor responses.
Developed using hybridoma or recombinant technologies, CD8a antibodies are widely employed in flow cytometry, immunohistochemistry (IHC), and functional assays. They help distinguish CD8+ T cells from CD4+ T cells, aiding studies on T cell differentiation, activation, and exhaustion. In cancer research, CD8a antibodies are used to quantify tumor-infiltrating lymphocytes (TILs), correlating CD8+ T cell density with prognosis or immunotherapy efficacy. They also facilitate functional analyses, such as cytotoxicity assays or cytokine profiling.
Key clones like SK1 (human) or 53-6.7 (mouse) are validated for specificity across applications. However, species cross-reactivity, epitope stability in fixed tissues, and compatibility with detection systems require optimization. Recent advancements include recombinant CD8a antibodies with enhanced consistency and minimal lot-to-lot variability, supporting standardized research and clinical diagnostics. These antibodies remain indispensable for dissecting CD8+ T cell biology in health and disease.
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