| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EDS4A; EDSVASC; PMGEDSV |
| Entrez GeneID | 1281 |
| clone | 8C1G11 |
| WB Predicted band size | 138.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human COL3A1 (AA: 24-153) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于COL3A1抗体的3篇代表性文献(模拟示例)：
1. **文献名称**：*"Immunohistochemical detection of COL3A1 in vascular pathology"*
**作者**：Smith A, et al.
**摘要**：通过免疫组化技术验证COL3A1抗体在血管壁中的特异性表达，揭示其在动脉瘤和血管发育异常中的异常分布。
2. **文献名称**：*"COL3A1 antibody validation for Western blot analysis in fibrotic disorders"*
**作者**：Lee J, et al.
**摘要**：评估多种商业COL3A1抗体的灵敏度和特异性，确定其在皮肤纤维化和肝纤维化组织样本Western blot中的可靠性。
3. **文献名称**：*"Role of COL3A1 in tumor microenvironment: Insights from immunofluorescence studies"*
**作者**：Garcia R, et al.
**摘要**：利用COL3A1抗体进行免疫荧光染色，证明III型胶原在肿瘤基质重塑中的作用，并关联其表达与患者预后。
4. **文献名称**：*"COL3A1 mutations and antibody-based detection in Ehlers-Danlos syndrome"*
**作者**：Chen L, et al.
**摘要**：结合基因测序和抗体检测，发现COL3A1突变导致胶原蛋白结构异常，为埃勒斯-当洛综合征的诊断提供双重验证方法。
(注：以上文献为模拟示例，实际引用需查询PubMed、Google Scholar等平台获取真实数据。)
The COL3A1 antibody targets the protein product of the COL3A1 gene, which encodes the alpha-1 chain of type III collagen—a major component of the extracellular matrix. Type III collagen is a fibrillar collagen predominantly found in skin, blood vessels, and internal organs, providing structural integrity and elasticity. It plays a critical role in tissue development, repair, and homeostasis. COL3A1 mutations are linked to vascular Ehlers-Danlos syndrome (vEDS), a rare connective tissue disorder characterized by fragile blood vessels and organ rupture.
COL3A1 antibodies are widely used in research to study collagen III expression, distribution, and abnormalities in diseases such as fibrosis, vascular pathologies, and inherited connective tissue disorders. In diagnostics, these antibodies help detect collagen III deficiencies or structural defects via immunohistochemistry (IHC), Western blotting, or immunofluorescence, aiding in vEDS confirmation. They also serve as tools to assess tissue remodeling in conditions like liver cirrhosis, pulmonary fibrosis, or wound healing.
Commercial COL3A1 antibodies are typically monoclonal or polyclonal, raised against specific epitopes of the collagen III alpha-1 chain. Validation includes reactivity checks in human/mouse tissues and comparison with knockout controls. Researchers must consider cross-reactivity with similar collagen types and optimize protocols for tissue-specific applications. Overall, COL3A1 antibodies are essential for advancing understanding of collagen-related diseases and developing therapeutic strategies.
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