| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MLH4; PMSL2; HNPCC4; PMS2CL |
| Entrez GeneID | 5395 |
| clone | 5A7F2 |
| WB Predicted band size | 95.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇关于 **PMS2抗体** 的参考文献及其简要摘要：
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1. **文献名称**：*"PMS2 immunohistochemistry in colorectal cancer: A practical review"*
**作者**：Kerr SE et al.
**摘要**：探讨PMS2抗体(克隆号EPR3947)在结直肠癌免疫组化中的临床应用，分析其在检测错配修复缺陷(dMMR)中的敏感性和特异性，强调需结合其他标志物(如MLH1)以提高诊断准确性。
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2. **文献名称**：*"Comparison of PMS2 antibody clones: Impact on Lynch syndrome screening"*
**作者**：Buchhorn HM et al.
**摘要**：比较不同PMS2抗体克隆(如EPR3947 vs. EPR3947(2))的性能，发现某些克隆在识别截短突变时存在差异，建议使用多克隆抗体或联合MLH1检测以减少假阴性结果。
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3. **文献名称**：*"Multiplex immunohistochemistry for PMS2 and MSH6 in endometrial cancer"*
**作者**：Le DT et al.
**摘要**：开发一种多重免疫组化方法，同时检测PMS2和MSH6表达，验证其在子宫内膜癌中筛查Lynch综合征的可行性，证明该方法可提高诊断效率并减少组织样本消耗。
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4. **文献名称**：*"PMS2 antibody specificity in hereditary cancer syndromes"*
**作者**：Huth C et al.
**摘要**：通过基因测序验证PMS2抗体(克隆号Abcam ab110638)的可靠性，发现其在遗传性肿瘤综合征(如Lynch综合征)中与基因突变高度一致，但需注意生殖细胞与体细胞突变的区分。
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这些研究聚焦于PMS2抗体的临床应用、克隆特异性、技术优化及其在遗传性肿瘤筛查中的作用。如需具体DOI或年份，可进一步补充说明!
The PMS2 antibody is a crucial tool in studying the PMS2 protein, a key component of the DNA mismatch repair (MMR) system. Encoded by the *PMS2* gene, this protein partners with MLH1 to form the MutLα complex, which identifies and corrects errors during DNA replication. Deficiencies in PMS2 disrupt MMR, leading to microsatellite instability (MSI) and an elevated risk of cancers, particularly Lynch syndrome-associated malignancies like colorectal and endometrial cancers.
PMS2 antibodies are widely used in immunohistochemistry (IHC) to assess PMS2 protein expression in tumor tissues, aiding in diagnosing Lynch syndrome and identifying sporadic cancers with MSI. However, PMS2 antibody specificity poses challenges due to structural homology with other post-meiotic segregation (PMS) family proteins and pseudogenes. Cross-reactivity, especially with PMS1. may occur, requiring careful validation and simultaneous testing of other MMR proteins (e.g., MLH1. MSH2. MSH6) for accurate interpretation.
Clinically, loss of PMS2 expression detected by these antibodies often guides genetic testing and personalized treatment, including immunotherapy for MSI-high tumors. Research applications include studying MMR mechanisms, carcinogenesis, and drug responses. Despite technical limitations, PMS2 antibodies remain vital in both diagnostic pathology and molecular oncology, underscoring their role in advancing precision medicine approaches for hereditary and sporadic cancers.
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