| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/500 - 1/2000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MLH4; PMSL2; HNPCC4; PMS2CL |
| Entrez GeneID | 5395 |
| clone | 8E4G11 |
| WB Predicted band size | 95.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于PMS2抗体的代表性文献，涵盖临床应用、抗体验证及标准化检测内容：
1. **标题**：*"Comparison of Monoclonal Antibodies Against PMS2 (EPR3947 and EP51) in Colorectal Cancer Diagnosis"*
**作者**：Bao F et al.
**摘要**：对比两种常用PMS2抗体(EPR3947与EP51)在结直肠癌免疫组化中的性能，发现EP51对核染色的特异性更高，尤其在林奇综合征筛查中假阳性率更低。
2. **标题**：*"PMS2 Immunohistochemistry in Endometrial Cancer: Pitfalls and Reproducibility"*
**作者**：Mills AM et al.
**摘要**：探讨子宫内膜癌中PMS2抗体检测的常见误区(如交叉反应、染色不均)，提出标准化染色流程以提高诊断一致性，强调需结合MLH1缺失分析。
3. **标题**：*"Validation of a Novel PMS2 Antibody for the Detection of Mismatch Repair Deficiency"*
**作者**：Shia J et al.
**摘要**：验证新型单克隆抗体(克隆号：A16-4)在微卫星不稳定肿瘤中的敏感性，证实其与基因测序结果高度吻合，适用于FFPE样本的临床检测。
注：以上文献信息为基于领域知识的模拟概括，实际引用需通过PubMed或学术数据库检索具体文章(可搜索关键词：PMS2 antibody, immunohistochemistry, Lynch syndrome)。近年研究多聚焦于抗体标准化及自动化检测优化。
The PMS2 antibody is a crucial diagnostic tool used in immunohistochemistry (IHC) to assess the expression of the PMS2 protein, a key component of the DNA mismatch repair (MMR) system. The PMS2 gene encodes a protein that partners with MLH1 to form the MutLα complex, which plays a vital role in correcting DNA replication errors by recognizing and repairing base-pair mismatches. Germline mutations in PMS2 are associated with Lynch syndrome, an inherited condition that increases the risk of colorectal, endometrial, and other cancers.
In clinical practice, PMS2 IHC staining helps identify MMR deficiency in tumor tissues. Loss of PMS2 protein expression often indicates a defect in the MMR pathway, which may arise from either somatic mutations or germline variants. Since PMS2 expression is dependent on MLH1. isolated loss of PMS2 (with preserved MLH1) typically points to a PMS2-specific mutation, while combined loss of PMS2 and MLH1 suggests an MLH1 aberration.
PMS2 antibodies are particularly valuable due to the gene's pseudogene homologs, which complicate genetic testing. IHC serves as a cost-effective screening method to guide confirmatory genetic analyses. However, interpretation requires caution, as weak or heterogeneous staining patterns may lead to false negatives. Current guidelines recommend combining PMS2 testing with other MMR proteins (MLH1. MSH2. MSH6) for comprehensive evaluation of Lynch syndrome and microsatellite instability status.
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