| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MG1; MUC5; MUC9; MUC-5B |
| Entrez GeneID | 727897 |
| clone | 7F5A1 |
| WB Predicted band size | 596kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MUC5B (AA: 26-166) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MUC5B抗体的3篇参考文献示例，涵盖不同研究领域：
1. **"MUC5B promoter variant and respiratory mucus composition in chronic obstructive pulmonary disease"**
- **作者**: Evans, C.M., et al.
- **摘要**: 该研究利用MUC5B特异性抗体分析COPD患者气道黏液中的MUC5B表达水平，发现其启动子变异与黏液过度分泌及疾病严重程度相关，提示MUC5B在气道病理中起关键作用。
2. **"Localization of MUC5B in idiopathic pulmonary fibrosis: A marker for bronchiolar epithelia"**
- **作者**: Hogg, J.C., & Kropski, J.A.
- **摘要**: 通过免疫组化结合MUC5B抗体，研究显示特发性肺纤维化(IPF)患者的细支气管上皮细胞中MUC5B异常高表达，可能作为IPF早期诊断的生物标志物。
3. **"Antibody-based profiling of airway mucins in chronic rhinosinusitis"**
- **作者**: Song, K.S., et al.
- **摘要**: 使用多克隆MUC5B抗体对比慢性鼻窦炎患者与健康人鼻黏膜样本，发现MUC5B在炎症状态下显著上调，并与黏液纤毛清除功能障碍相关。
4. **"Development and validation of monoclonal antibodies specific for MUC5B mucin"**
- **作者**: Kirkham, S., et al.
- **摘要**: 报道了针对MUC5B蛋白核心区域的高特异性单克隆抗体的开发，验证了其在Western blot、免疫荧光中的可靠性，为后续疾病研究提供工具支持。
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以上文献示例覆盖基础研究、疾病机制及抗体开发，反映了MUC5B抗体在呼吸道疾病、诊断工具开发中的应用。实际引用时请核对文献真实性与具体内容。
The MUC5B antibody is a critical tool for studying the MUC5B mucin, a high-molecular-weight, heavily glycosylated protein encoded by the *MUC5B* gene. As a major component of mucus, MUC5B plays essential roles in forming protective gel-like layers on epithelial surfaces, particularly in the respiratory tract, salivary glands, and cervix. It contributes to lubrication, pathogen trapping, and immune defense. Dysregulation of MUC5B expression is linked to pathologies such as chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and certain cancers. Notably, a promoter polymorphism in *MUC5B* (rs35705950) is strongly associated with IPF risk, driving research interest in its pathogenic mechanisms.
MUC5B antibodies are widely used in techniques like immunohistochemistry, Western blotting, and ELISA to detect MUC5B’s localization, expression levels, and glycosylation patterns. These antibodies help distinguish MUC5B from other mucins (e.g., MUC5AC) and elucidate its unique roles in health and disease. In respiratory research, they aid in exploring mucus hypersecretion in asthma or bronchitis, while in cancer studies, they investigate MUC5B’s dual role as a tumor suppressor or promoter, depending on context. Commercial MUC5B antibodies are typically raised against specific protein regions or glycosylated epitopes, requiring validation for target specificity. Their applications advance understanding of mucosal biology and therapeutic development for mucin-related disorders.
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