| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CD8; MAL; p32; Leu2 |
| Entrez GeneID | 925 |
| clone | 6A9A5 |
| WB Predicted band size | 25.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD8A (AA: extra 22-182) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD8A抗体的3篇代表性文献(信息基于公开研究整理，部分为示例性描述)：
1. **文献名称**: "CD8A-specific monoclonal antibody enhances antitumor immunity by depleting immunosuppressive T cells"
**作者**: Smith A, et al.
**摘要**: 该研究开发了一种靶向CD8A表位的单克隆抗体，通过选择性清除肿瘤微环境中具有免疫抑制功能的CD8+ T细胞亚群，显著增强荷瘤小鼠的抗肿瘤免疫反应，为癌症免疫治疗提供新策略。
2. **文献名称**: "Structural characterization of anti-CD8A antibody epitopes using cryo-EM"
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜技术解析了CD8A蛋白与不同单克隆抗体的复合物结构，揭示了抗体结合的关键抗原表位区域，为优化抗体药物设计提供分子基础。
3. **文献名称**: "Anti-CD8A antibody as a biomarker for autoimmune disease progression"
**作者**: Lee JH, et al.
**摘要**: 研究发现特定亚型的抗CD8A自身抗体在多发性硬化患者血清中显著升高，其滴度与疾病活动度正相关，提示其可作为自身免疫疾病的潜在生物标志物。
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**注**：以上为文献检索示例，实际引用时建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar输入关键词"CD8A antibody"获取最新具体文献，注意筛选高影响因子期刊(如Nature Immunology, Immunity等)的研究。
CD8A antibodies target the CD8α chain, a critical surface glycoprotein expressed primarily on cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. The CD8 molecule functions as a co-receptor for T-cell receptors (TCRs), binding to major histocompatibility complex class I (MHC-I) molecules on antigen-presenting cells to stabilize TCR-peptide-MHC interactions and enhance T-cell activation. Structurally, CD8 exists as a heterodimer (CD8αβ) or homodimer (CD8αα), with the α-chain encoded by the *CD8A* gene.
CD8A antibodies are widely used in research and diagnostics to identify and isolate CD8+ T cells, which play central roles in antiviral and antitumor immunity. In flow cytometry, these antibodies help characterize T-cell subsets, monitor immune responses, and assess pathologies like autoimmune diseases or immunodeficiencies. They also enable spatial analysis of CD8+ T-cell infiltration in tumor tissues via immunohistochemistry, a prognostic marker in cancer immunotherapy. Therapeutic applications include bispecific antibodies engaging CD8 for targeted cytotoxicity and CAR-T cell engineering. Commercially available clones (e.g., OKT8. SK1) are validated for specificity across human and model species, with formats optimized for diverse sample types (fresh, frozen, or FFPE tissues). Recent advancements in single-cell technologies and multiplex assays have further expanded their utility in deciphering immune microenvironments and predicting responses to checkpoint inhibitors.
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