| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 2549 |
| clone | 1A7 |
| WB Predicted band size | 76.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GAB1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GAB1抗体的3篇参考文献示例(注：内容为模拟虚构，仅供参考)：
1. **文献名称**: "GAB1 Antibody Specificity and Application in Hepatocellular Carcinoma Studies"
**作者**: Li, X. et al.
**摘要**: 该研究验证了GAB1抗体在Western blot和免疫组化中的特异性，发现其在肝癌组织中高表达，并与患者预后不良相关。实验表明GAB1通过调控MAPK/ERK通路促进肿瘤侵袭。
2. **文献名称**: "Role of GAB1 in EGFR Signaling Revealed by Targeted Antibody Blockade"
**作者**: Müller, J. & Schmidt, A.
**摘要**: 通过使用特异性GAB1抗体阻断实验，证明GAB1在EGFR信号转导中与Grb2形成复合物，调控细胞增殖和迁移，为靶向治疗提供潜在策略。
3. **文献名称**: "Development of a Novel Monoclonal GAB1 Antibody for Cardiac Development Research"
**作者**: Chen, Y. et al.
**摘要**: 研究团队开发了一种高灵敏度单克隆GAB1抗体，成功应用于斑马鱼模型，揭示GAB1在心脏发育中通过PI3K-Akt通路调控心肌细胞分化的机制。
(注：以上文献信息为示例性内容，实际引用需查询PubMed等数据库获取真实文献。)
GAB1 (GRB2-associated binding protein 1) is a scaffolding/adaptor protein critical for signal transduction downstream of receptor tyrosine kinases (RTKs) and cytokine receptors. It belongs to the GAB/DOS family and contains multiple functional domains, including a pleckstrin homology (PH) domain that binds phosphatidylinositol lipids, and tyrosine phosphorylation sites that recruit signaling molecules like PI3K, SHP2. and CRKL. GAB1 facilitates the activation of key pathways such as PI3K/AKT and RAS/MAPK, regulating cell proliferation, survival, migration, and differentiation.
GAB1 antibodies are essential tools for studying its role in cellular signaling. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect GAB1 expression, phosphorylation status, and protein-protein interactions. These antibodies help researchers explore GAB1's involvement in physiological processes and diseases, particularly cancer, where GAB1 overexpression or dysregulation is linked to tumor progression and metastasis. For example, aberrant GAB1 signaling is associated with breast, lung, and liver cancers. Additionally, GAB1 antibodies may aid in understanding developmental disorders and immune responses, as GAB1 interacts with MET, EGFR, and other receptors implicated in tissue morphogenesis and inflammation. Their specificity and reliability make GAB1 antibodies valuable for both basic research and potential clinical applications in diagnostics or therapeutic monitoring.
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