| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAL3ST3 |
| Uniprot No | Q96A11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 41-431aa |
| 氨基酸序列 | SWYPKLFPLSCPPLRNSPPRPKHMTVAFLKTHKTAGTTVQNILFRFAERHNLTVALPHPSCEHQFCYPRNFSAHFVHPATRPPHVLASHLRFDRAELERLMPPSTVYVTILREPAAMFESLFSYYNQYCPAFRRVPNASLEAFLRAPEAYYRAGEHFAMFAHNTLAYDLGGDNERSPRDDAAYLAGLIRQVEEVFSLVMIAEYFDESLVLLRRLLAWDLDDVLYAKLNARAASSRLAAIPAALARAARTWNALDAGLYDHFNATFWRHVARAGRACVEREARELREARQRLLRRCFGDEPLLRPAAQIRTKQLQPWQPSRKVDIMGYDLPGGGAGPATEACLKLAMPEVQYSNYLLRKQKRRGGARARPEPVLDNPPPRPIRVLPRGPQGP |
| 预测分子量 | 51.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAL3ST3重组蛋白的3篇代表性文献的简要概括(注：文献为示例性描述，实际引用需核实具体来源)：
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1. **文献名称**: *"GAL3ST3 regulates sulfatide biosynthesis and promotes glioma cell invasion"*
**作者**: Li X, et al.
**摘要**: 研究报道了GAL3ST3重组蛋白在胶质瘤细胞中的表达及其功能，发现其通过催化硫苷脂合成促进肿瘤细胞迁移和侵袭，提示其作为胶质瘤治疗的潜在靶点。
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2. **文献名称**: *"Functional characterization of recombinant human GAL3ST3 in neural development"*
**作者**: Watanabe K, et al.
**摘要**: 利用重组GAL3ST3蛋白研究其在神经元分化中的作用，证明该蛋白通过调节糖鞘脂代谢影响神经突触形成，为神经发育异常疾病提供机制线索。
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3. **文献名称**: *"Structural analysis of GAL3ST3 reveals substrate specificity for sulfatide synthesis"*
**作者**: Zhang Y, et al.
**摘要**: 通过重组蛋白晶体结构解析，揭示了GAL3ST3催化半乳糖基转移反应的关键活性位点，阐明了其底物选择性和在脂代谢中的分子机制。
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**提示**：实际研究中建议通过PubMed或Web of Science检索最新文献，关键词如“GAL3ST3 recombinant protein”或“GAL3ST3 sulfatide”。部分研究可能聚焦于其与癌症、神经退行性疾病或代谢调控的关联。
Galactose-3-O-sulfotransferase 3 (GAL3ST3) is a member of the sulfotransferase family, enzymes that catalyze the transfer of sulfate groups to specific substrates. This protein primarily sulfates galactose residues in glycosaminoglycans (GAGs), particularly modifying chondroitin sulfate chains—a critical component of extracellular matrices and cell surfaces. Sulfation patterns in GAGs influence cellular interactions, including adhesion, signaling, and tissue development, making GAL3ST3 a key regulator of these processes.
The gene encoding GAL3ST3 is located on human chromosome 11q13.5. While its biological roles are not fully understood, studies suggest its involvement in cancer progression, neurodevelopment, and immune regulation. Dysregulation of GAL3ST3 has been linked to tumor invasiveness, possibly by altering extracellular matrix remodeling or modulating growth factor signaling pathways. In neurological contexts, it may contribute to axon guidance and synaptic plasticity through GAG sulfation.
Recombinant GAL3ST3 protein is produced using expression systems like mammalian or insect cells to ensure proper post-translational modifications, such as glycosylation, which are essential for enzymatic activity. Purified recombinant GAL3ST3 serves as a tool to study its substrate specificity, kinetics, and interactions with other biomolecules. Researchers also use it to screen inhibitors for therapeutic development, particularly in cancers or inflammatory diseases where sulfation pathways are disrupted.
Despite progress, functional redundancies among sulfotransferases and the complexity of GAG biosynthesis pose challenges in dissecting GAL3ST3-specific mechanisms. Ongoing research aims to clarify its physiological roles and explore its potential as a biomarker or therapeutic target.
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