| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIF1 |
| Uniprot No | Q13105 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-803aa |
| 氨基酸序列 | MDFPQHSQHVLEQLNQQRQLGLLCDCTFVVDGVHFKAHKAVLAACSEYFKMLFVDQKDVVHLDISNAAGLGQVLEFMYTAKLSLSPENVDDVLAVATFLQMQDIITACHALKSLAEPATSPGGNAEALATEGGDKRAKEEKVATSTLSRLEQAGRSTPIGPSRDLKEERGGQAQSAASGAEQTEKADAPREPPPVELKPDPTSGMAAAEAEAALSESSEQEMEVEPARKGEEEQKEQEEQEEEGAGPAEVKEEGSQLENGEAPEENENEESAGTDSGQELGSEARGLRSGTYGDRTESKAYGSVIHKCEDCGKEFTHTGNFKRHIRIHTGEKPFSCRECSKAFSDPAACKAHEKTHSPLKPYGCEECGKSYRLISLLNLHKKRHSGEARYRCEDCGKLFTTSGNLKRHQLVHSGEKPYQCDYCGRSFSDPTSKMRHLETHDTDKEHKCPHCDKKFNQVGNLKAHLKIHIADGPLKCRECGKQFTTSGNLKRHLRIHSGEKPYVCIHCQRQFADPGALQRHVRIHTGEKPCQCVMCGKAFTQASSLIAHVRQHTGEKPYVCERCGKRFVQSSQLANHIRHHDNIRPHKCSVCSKAFVNVGDLSKHIIIHTGEKPYLCDKCGRGFNRVDNLRSHVKTVHQGKAGIKILEPEEGSEVSVVTVDDMVTLATEALAATAVTQLTVVPVGAAVTADETEVLKAEISKAVKQVQEEDPNTHILYACDSCGDKFLDANSLAQHVRIHTAQALVMFQTDADFYQQYGPGGTWPAGQVLQAGELVFRPRDGAEGQPALAETSPTAPECPPPAE |
| 预测分子量 | 87,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GIF1重组蛋白的参考文献示例(注：部分文献为假设性示例，实际文献需通过学术数据库核实)：
1. **标题**: *"Heterologous Expression and Functional Analysis of Recombinant GIF1 in Regulating Plant Growth"*
**作者**: Chen L, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了植物源GIF1重组蛋白，证实其通过调控细胞伸长影响拟南芥叶片发育，为植物生长调控机制提供了新见解。
2. **标题**: *"Recombinant GIF1 Protein Enhances Insulin Secretion in Pancreatic β-Cells via cAMP Signaling"*
**作者**: Kim S, et al.
**摘要**: 利用哺乳动物细胞系统表达人源GIF1重组蛋白，发现其通过激活cAMP通路促进胰岛素分泌，提示其在糖尿病治疗中的潜在应用价值。
3. **标题**: *"Crystal Structure and Biochemical Characterization of Human GIF1 Recombinant Protein"*
**作者**: Patel R, et al.
**摘要**: 解析了人源GIF1重组蛋白的高分辨率晶体结构，结合体外结合实验，揭示了其与胃肠激素受体的特异性互作位点。
4. **标题**: *"Optimization of GIF1 Recombinant Protein Production in Pichia pastoris for Therapeutic Development"*
**作者**: González M, et al.
**摘要**: 在毕赤酵母中优化GIF1重组蛋白的分泌表达工艺，获得高产量活性蛋白，并验证其在小鼠模型中的代谢调节功效。
**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台以“GIF1 recombinant protein”或结合具体研究领域(如“GIF1 glucose metabolism”)为关键词检索最新文献。
**Background of GIF1 Recombinant Protein**
GIF1 (Gastric Intrinsic Factor 1) is a glycoprotein critical for the absorption of vitamin B12 (cobalamin) in humans. Produced by parietal cells in the gastric mucosa, native GIF binds to dietary vitamin B12 in the stomach, forming a complex that is recognized by receptors in the distal ileum, enabling intestinal uptake. Deficiency in GIF, often due to autoimmune disorders or gastric atrophy, leads to pernicious anemia and neurological complications due to impaired B12 absorption.
Recombinant GIF1 protein is engineered using biotechnological methods, typically expressed in mammalian or bacterial systems to ensure proper folding and post-translational modifications. Its production addresses challenges in studying and treating GIF-related deficiencies, offering a standardized and scalable source of the protein. Researchers utilize recombinant GIF1 to investigate its structural-functional relationships, receptor interactions, and role in B12 metabolism. It also holds therapeutic potential, such as in oral supplementation strategies to bypass defective intrinsic factor in patients.
Additionally, recombinant GIF1 serves as a tool in diagnostic assays to detect autoimmune antibodies against intrinsic factor, aiding in the diagnosis of pernicious anemia. Advances in protein engineering and expression systems continue to optimize its stability and bioavailability, broadening its applications in both clinical and research settings. The development of GIF1 recombinant protein underscores the intersection of molecular biology and medical innovation in addressing nutrient absorption disorders.
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