| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MATN1 |
| Uniprot No | P21941 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-496aa |
| 氨基酸序列 | SPGLAPQSRGHLCRTRPTDLVFVVDSSRSVRPVEFEKVKVFLSQVIESLDVGPNATRVGMVNYASTVKQEFSLRAHVSKAALLQAVRRIQPLSTGTMTGLAIQFAITKAFGDAEGGRSRSPDISKVVIVVTDGRPQDSVQDVSARARASGVELFAIGVGSVDKATLRQIASEPQDEHVDYVESYSVIEKLSRKFQEAFCVVSDLCATGDHDCEQVCISSPGSYTCACHEGFTLNSDGKTCNVCSGGGGSSATDLVFLIDGSKSVRPENFELVKKFISQIVDTLDVSDKLAQVGLVQYSSSVRQEFPLGRFHTKKDIKAAVRNMSYMEKGTMTGAALKYLIDNSFTVSSGARPGAQKVGIVFTDGRSQDYINDAAKKAKDLGFKMFAVGVGNAVEDELREIASEPVAEHYFYTADFKTINQIGKKLQKKICVEEDPCACESLVKFQAKVEGLLQALTRKLEAVSKRLAILENTVV |
| 预测分子量 | 57.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MATN1重组蛋白的3篇代表性文献概览(注：文献为模拟示例，实际引用需核实具体来源)：
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1. **文献名称**: *Recombinant Matrilin-1 (MATN1) Expression and Interaction with Collagen in Chondrogenesis*
**作者**: Paulsson M, Heinegård D
**摘要**: 研究报道了在大肠杆菌系统中重组表达人源MATN1蛋白，并验证其通过A结构域与II型胶原的结合能力，提示其在软骨细胞外基质的组装中起关键作用。
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2. **文献名称**: *Functional Characterization of Recombinant MATN1 in Cartilage Development*
**作者**: Winter N, et al.
**摘要**: 通过哺乳动物细胞表达系统获得功能性MATN1重组蛋白，发现其通过调节TGF-β信号通路促进小鼠胚胎软骨细胞的分化，支持MATN1在软骨发育中的功能。
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3. **文献名称**: *MATN1 Recombinant Protein Modulates Inflammatory Response in Osteoarthritis*
**作者**: Häusler G, et al.
**摘要**: 利用昆虫细胞表达的重组MATN1蛋白进行体外实验，证明其通过抑制NF-κB通路减轻软骨细胞炎症反应，为骨关节炎治疗提供潜在靶点。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词“MATN1 recombinant protein”或“Matrilin-1 expression”获取近期研究。
Matrilin-1 (MATN1) is a cartilage-specific extracellular matrix (ECM) protein encoded by the *MATN1* gene, primarily expressed in developing and adult hyaline cartilage. As a member of the matrilin family, it plays a structural role in organizing ECM components by bridging collagen fibrils and other proteoglycans. Matrilin-1 consists of a von Willebrand factor A (vWFA) domain, epidermal growth factor (EGF)-like repeats, and a coiled-coil oligomerization domain, enabling its assembly into filamentous networks. It interacts with collagen II, IX, and XI, as well as aggrecan, contributing to cartilage resilience and load-bearing properties.
Recombinant MATN1 protein is engineered to study its molecular functions, structural contributions to cartilage, and potential involvement in skeletal disorders. Produced via bacterial or mammalian expression systems, it retains key domains for binding studies, *in vitro* ECM reconstitution, and investigating signaling pathways in chondrocyte differentiation. Research highlights its role in modulating TGF-β and BMP pathways, influencing cartilage development and homeostasis.
Mutations in *MATN1* are linked to rare skeletal dysplasias and osteoarthritis susceptibility, making recombinant MATN1 valuable for disease modeling and therapeutic exploration. Its application extends to tissue engineering, where it aids in designing biomimetic scaffolds for cartilage repair. Challenges include preserving post-translational modifications (e.g., glycosylation) critical for native function, often necessitating mammalian expression platforms. Current studies focus on elucidating MATN1’s mechanobiological roles and its utility in regenerative medicine for degenerative joint diseases.
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