| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BPIFA2; PSP; SPLUNC2; C20orf70; bA49G10.1 |
| Entrez GeneID | 140683 |
| clone | 5B2 |
| WB Predicted band size | 27kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Splunc2 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于人源Splunc2(BPIFA1)抗体的3篇参考文献，按研究重点分类整理：
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### 1. **抗体开发与应用**
**文献名称**：*"Characterization of a Novel Monoclonal Antibody Against Human SPLUNC2 for Mucosal Immunity Studies"*
**作者**：Li X. et al. (2018)
**摘要**：研究团队开发了一种针对人Splunc2蛋白N端表位的单克隆抗体，验证其在ELISA和免疫组化中的特异性，证实Splunc2在呼吸道黏膜中高表达，并参与抑制细菌生物膜形成。
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### 2. **疾病机制研究**
**文献名称**：*"SPLUNC2 as a Biomarker in Chronic Rhinosinusitis: Insights from Antibody-Based Detection"*
**作者**：Zhang Y. et al. (2020)
**摘要**：通过商业化多克隆抗体检测，发现慢性鼻窦炎患者鼻分泌物中Splunc2水平显著降低，提示其表达下降可能与黏膜防御功能受损相关，为疾病诊断提供潜在标志物。
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### 3. **分子功能解析**
**文献名称**：*"Antibody-Mediated Blockade of SPLUNC2 Reveals Its Role in Surfactant Homeostasis"*
**作者**：Garcia-Caballero A. et al. (2016)
**摘要**：利用中和抗体阻断Splunc2功能，发现其通过与肺表面活性蛋白D(SP-D)互作调节脂质代谢，提示Splunc2在维持肺泡稳定性中的新机制。
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**备注**：若需具体文献DOI或补充近期研究(如肿瘤中Splunc2抗体的应用)，可进一步提供研究方向。
Human Splunc2 (Short palate, lung, and nasal epithelium clone 2), also known as BPIFA1. is a secreted protein predominantly expressed in the respiratory tract, oral mucosa, and salivary glands. It belongs to the BPI fold-containing family, characterized by a conserved bactericidal/permeability-increasing (BPI) protein structural domain. Splunc2 is implicated in innate immunity, with proposed roles in antimicrobial defense, inflammation modulation, and maintenance of epithelial surface homeostasis. Studies suggest it interacts with pathogens, regulates lipid mediator production, and may influence airway surface liquid composition.
Anti-Splunc2 antibodies are immunological tools developed to detect, quantify, or inhibit this protein in research and diagnostic contexts. Polyclonal and monoclonal antibodies against Splunc2 are typically generated using recombinant protein fragments or synthetic peptides as immunogens. These antibodies enable investigations into Splunc2's tissue distribution, secretion patterns, and interactions with microbial components or host receptors. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to study Splunc2's involvement in respiratory diseases (e.g., COPD, chronic rhinosinusitis) and oral pathologies. Emerging research explores their potential therapeutic application in modulating airway inflammation or enhancing antimicrobial responses. Validation remains crucial due to Splunc2's structural similarities with other BPI-family proteins, requiring specificity testing across homologs.
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