| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FKH1; FKHR; FOXO1A |
| Entrez GeneID | 2308 |
| clone | 3B6 |
| WB Predicted band size | 69.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit,Monkey |
| Immunogen | Purified recombinant fragment of human FOXO1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇关于FOXO1抗体的参考文献，按文献名称、作者和摘要内容简要概括：
1. **"FOXO1 regulates lysosomal expression and lipid metabolism in macrophages"**
- **作者**: Webb AE et al.
- **摘要**: 研究通过FOXO1特异性抗体(ChIP-seq和Western blot)发现FOXO1直接调控巨噬细胞溶酶体相关基因表达，影响脂质代谢和动脉粥样硬化进程。
2. **"Phosphorylation and regulation of FOXO1 by Akt/PKB in hepatic gluconeogenesis"**
- **作者**: Altomonte J et al.
- **摘要**: 利用FOXO1磷酸化特异性抗体，揭示胰岛素/Akt信号通过磷酸化FOXO1抑制其核转位，从而调控肝脏糖异生的分子机制。
3. **"FOXO1 mediates resistance to tumor angiogenesis inhibition in ovarian cancer"**
- **作者**: Xiang T et al.
- **摘要**: 通过免疫组化(FOXO1抗体)和基因敲除实验，证明FOXO1在卵巢癌中通过调控VEGF表达促进肿瘤血管生成，并与抗血管治疗耐药性相关。
4. **"Tissue-specific regulation of FOXO1 by Sirt1 in metabolic homeostasis"**
- **作者**: Frescas D et al.
- **摘要**: 使用FOXO1抗体进行组织染色和共沉淀实验，发现Sirt1通过去乙酰化修饰FOXO1.影响脂肪和肝脏组织的代谢平衡及胰岛素敏感性。
以上研究均通过FOXO1抗体在分子机制或疾病模型中验证其功能，涵盖代谢、癌症及表观遗传调控等领域。
The FOXO1 antibody is a crucial tool in studying the Forkhead box O1 (FOXO1) protein, a member of the evolutionarily conserved FOXO transcription factor family. FOXO1 plays a pivotal role in regulating cellular processes such as apoptosis, autophagy, cell cycle arrest, and stress resistance. It acts as a downstream effector of the insulin/PI3K/AKT signaling pathway, where AKT-mediated phosphorylation triggers its cytoplasmic sequestration and inactivation. Dysregulation of FOXO1 is implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders.
FOXO1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation at Ser256 or Thr24) through techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate FOXO1's tumor-suppressive roles in certain cancers or its context-dependent oncogenic functions. In metabolic studies, they aid in exploring FOXO1's regulation of gluconeogenesis in the liver and its interaction with PPARγ in adipocyte differentiation.
Commercial FOXO1 antibodies are typically raised against specific epitopes, with validation in knockout controls being critical due to potential cross-reactivity with other FOXO family members (FOXO3. FOXO4. FOXO6). Their applications span basic research, drug development, and diagnostic investigations into diseases linked to insulin signaling and oxidative stress pathways.
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