| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAPC1 |
| Uniprot No | P25858 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-338aa |
| 氨基酸序列 | MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKHNELKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHSITATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYDEIKKAIKEESEGKLKGILGYTEDDVVSTDFVGDNRSSIFDAKAGIALSDKFVKLVSWYDNEWGYSSRVVDLIVHMSKA |
| 预测分子量 | 44.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAPC1重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**：*Recombinant expression and kinetic characterization of cytoplasmic GAPC1 from Arabidopsis thaliana*
**作者**：Muñoz-Bertomeu, J., et al.
**摘要**：研究在大肠杆菌中重组表达了拟南芥胞质GAPC1蛋白，通过酶动力学分析证实其催化甘油醛-3-磷酸转化为1.3-二磷酸甘油酸的能力，并探讨其在植物糖酵解中的核心作用。
2. **文献名称**：*GAPC1 interacts with mitochondrial proteins to coordinate cellular energy metabolism under stress conditions*
**作者**：Guo, L., et al.
**摘要**：利用重组GAPC1蛋白进行体外互作实验，发现其在氧化胁迫下与线粒体抗坏血酸过氧化物酶(APX)结合，提示GAPC1可能通过调节活性氧代谢参与胁迫响应。
3. **文献名称**：*Non-glycolytic roles of recombinant plant GAPC1: Involvement in DNA damage repair*
**作者**：Rius, S.P., et al.
**摘要**：通过重组拟南芥GAPC1蛋白的功能实验，发现其除糖酵解功能外，还能在体外与受损DNA结合，暗示其在核内可能参与DNA修复过程。
---
注：以上文献为示例性质，实际引用时建议通过学术数据库(如PubMed、Web of Science)核对最新研究。如需具体文章链接或补充信息，可进一步说明。
**Background of GAPC1 Recombinant Protein**
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a highly conserved enzyme central to glycolysis, catalyzing the conversion of glyceraldehyde-3-phosphate to 1.3-bisphosphoglycerate. While traditionally associated with energy metabolism, GAPDH has multifaceted roles in cellular processes, including DNA repair, apoptosis, and transcriptional regulation. GAPC1 (GAPDH Cytosolic 1) refers to a specific cytosolic isoform of GAPDH, predominantly studied in plants, where it contributes to both metabolic and stress-response pathways.
Recombinant GAPC1 protein is engineered through molecular cloning, where the *GAPC1* gene is inserted into expression vectors (e.g., *E. coli* or yeast systems) for large-scale production. This allows researchers to obtain purified, functional GAPC1 for structural, biochemical, and functional studies. The recombinant form retains enzymatic activity and is critical for investigating GAPDH's non-canonical roles, such as its interaction with nucleic acids or proteins under oxidative stress.
In plants, GAPC1 is implicated in abiotic stress responses (e.g., drought, salinity) by modulating redox homeostasis and signaling pathways. Its recombinant version enables detailed studies on post-translational modifications (e.g., S-nitrosylation) that regulate its dual metabolic and signaling functions. Additionally, GAPC1 recombinant protein serves as a tool in biomedical research, given GAPDH's links to neurodegenerative diseases, cancer, and microbial pathogenesis.
Overall, GAPC1 recombinant protein bridges gaps in understanding the enzyme's evolutionary conservation, multifunctionality, and therapeutic potential, making it a valuable resource across plant biology, biochemistry, and disease research.
×
