Mouse Monoclonal RAF1 Antibody

The RAF1 antibody is a crucial tool in studying the RAF1 protein, a serine/threonine kinase central to the MAPK/ERK signaling pathway. RAF1 (also known as CRAF) plays a pivotal role in regulating cell growth, differentiation, and survival by transmitting signals from RAS GTPases to MEK and ERK kinases. Dysregulation of RAF1 is implicated in various cancers, developmental disorders (e.g., Noonan syndrome), and other diseases, often due to mutations or aberrant activation. RAF1 antibodies are widely used in research to detect RAF1 expression, phosphorylation status, and interactions via techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP). Specific antibodies can distinguish between inactive and active (phosphorylated) forms, aiding in studies of pathway activation. Additionally, they help evaluate therapeutic responses to RAF or MEK inhibitors in preclinical models. Challenges include ensuring specificity, as RAF1 shares homology with other RAF family members (ARAF, BRAF), and optimizing conditions for different sample types. Commercial RAF1 antibodies are typically developed in hosts like rabbits or mice, targeting epitopes in conserved regions (e.g., N-terminal regulatory or C-terminal kinase domains). Validation via knockout controls or siRNA knockdown is critical to confirm reliability. Overall, RAF1 antibodies remain indispensable for unraveling the protein's role in health and disease.