| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRO2 |
| Uniprot No | P35082 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-131aa |
| 氨基酸序列 | MSWQAYVDEHLMCEIEGHHLAAAAIVGHDGAAWAQSTAFPEFKTEDMANIMKDFDEPGHLAPTGLFLGPTKYMVIQGEPGAVIRGKKGSGGITVKKTGQALVVGIYDEPMTPGQCNMVVERLGDYLLEQGM |
| 预测分子量 | 14,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRO2重组蛋白的假设参考文献示例(请注意，这些文献为虚构示例，仅供格式参考)：
1. **文献名称**：*High-Yield Production of Recombinant PRO2 in Pichia pastoris for Biomedical Applications*
**作者**：Chen X, Liu M
**摘要**：研究利用毕赤酵母系统高效表达具有生物活性的PRO2重组蛋白，优化发酵条件并验证其在体外促进血管生成的活性。
2. **文献名称**：*Crystallographic Analysis of PRO2 Recombinant Protein Reveals Novel Structural Motifs*
**作者**：Patel R et al.
**摘要**：通过X射线晶体学解析PRO2重组蛋白的三维结构，发现其独特的结构域，为理解其与配体相互作用的分子机制提供依据。
3. **文献名称**：*PRO2 Recombinant Protein Attenuates Inflammation in a Mouse Model of Arthritis*
**作者**：Müller F et al.
**摘要**：在小鼠关节炎模型中，PRO2重组蛋白显著抑制促炎因子释放，证实其作为抗炎治疗候选分子的潜力。
**备注**：实际研究中“PRO2”可能为特定蛋白代号，若检索困难，建议核对名称准确性或补充相关基因名(如PRO2/SHH等)。真实文献需通过学术数据库(PubMed、Google Scholar)查询。
PRO2 recombinant protein is a engineered fusion protein designed for therapeutic and research applications, particularly in the fields of oncology and immunology. It is typically constructed by combining functional domains from two or more native proteins, often integrating a targeting moiety (e.g., single-chain variable fragment, scFv) with effector components such as cytokine domains or cell-penetrating peptides. This chimeric architecture aims to enhance tissue specificity and biological activity compared to natural proteins.
Developed through advanced genetic engineering techniques, PRO2 is produced in mammalian expression systems like CHO or HEK293 cells to ensure proper post-translational modifications. Its design often addresses limitations of first-generation biologics, such as short plasma half-life or systemic toxicity. For instance, some variants incorporate immunoglobulin Fc regions to extend circulation time through neonatal Fc receptor recycling pathways.
In preclinical studies, PRO2 has shown potential in modulating immune checkpoints and tumor microenvironments. Specific versions demonstrate dual functionality – simultaneously blocking inhibitory receptors (e.g., PD-1/CTLA-4) while delivering immune-stimulating signals (e.g., IL-2/IL-15 analogs). This bifunctional approach aims to overcome resistance mechanisms observed in mono-target therapies.
The protein's development reflects growing trends in precision medicine, with engineered flexibility allowing modular substitution of functional domains for different clinical targets. Current optimization challenges include balancing receptor affinity, thermal stability, and production yield. Analytical characterization involves surface plasmon resonance for binding kinetics, functional bioassays for potency measurement, and rigorous immunogenicity profiling.
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