| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L |
| Entrez GeneID | 6500 |
| clone | 1H9 |
| WB Predicted band size | 19kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SKP1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SKP1抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*"SKP1 regulates cell cycle progression through ubiquitin-mediated degradation of cyclin-dependent kinase inhibitors"*
**作者**：Zhang Y, et al.
**摘要**：本研究利用特异性SKP1抗体，通过免疫沉淀和Western blot分析，揭示了SKP1在SCF复合体中的核心作用，证明其通过泛素化降解CDK抑制剂(如p27)调控细胞周期进程。
2. **文献名称**：*"Development and validation of a high-affinity monoclonal antibody for SKP1 in cancer tissue profiling"*
**作者**：Lee S, et al.
**摘要**：文章报道了一种新型SKP1单克隆抗体的开发，验证了其在免疫组化中的特异性，并发现SKP1在多种癌症组织(如乳腺癌、结肠癌)中高表达，提示其作为肿瘤标志物的潜力。
3. **文献名称**：*"SKP1-Cullin-F-box interactions: Structural insights revealed by cross-linking and antibody-based assays"*
**作者**：Thompson R, et al.
**摘要**：通过交联实验结合SKP1抗体介导的共免疫沉淀，阐明了SKP1与Cullin及F-box蛋白的动态结合机制，为靶向SCF复合体的药物设计提供了结构基础。
(注：以上文献信息为模拟生成，实际引用请以真实论文为准。)
SKP1 (S-phase kinase-associated protein 1) is a core component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which plays a crucial role in the ubiquitin-proteasome system by targeting specific proteins for degradation. This complex regulates diverse cellular processes, including cell cycle progression, signal transduction, and transcriptional regulation. SKP1 acts as an adaptor protein, bridging F-box proteins (substrate receptors) to CUL1 (Cullin 1), thereby facilitating substrate recognition and polyubiquitination. Dysregulation of SKP1 or SCF complexes has been implicated in cancer, neurodegenerative diseases, and immune disorders.
SKP1 antibodies are essential tools for studying the expression, localization, and function of SKP1 in various biological contexts. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate SKP1's interaction partners, post-translational modifications, and involvement in disease pathways. Commercially available SKP1 antibodies are typically raised against conserved epitopes, ensuring cross-reactivity in human, mouse, and rat samples. Validation of these antibodies includes testing for specificity via knockout cell lines or siRNA knockdown. Research utilizing SKP1 antibodies has advanced our understanding of ubiquitin-mediated proteolysis mechanisms and their therapeutic targeting in pathological conditions.
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