| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NEDD-8 |
| Entrez GeneID | 4738 |
| clone | 1A7 |
| WB Predicted band size | 9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NEDD8 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3条关于NEDD8抗体的参考文献及其摘要内容：
1. **文献名称**：*NEDD8. a Novel Ubiquitin-like Protein that Interacts with NEDD8-conjugating Enzyme Ubc12*
**作者**：Kamitani T, Kito K, Fukuda-Kamitani T, Yeh ET
**摘要**：该研究首次克隆并鉴定了NEDD8蛋白，开发了特异性抗体，证明其通过Ubc12酶与靶蛋白结合，参与细胞周期调控。抗体成功用于检测内源性NEDD8及其修饰蛋白。
2. **文献名称**：*An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer*
**作者**：Soucy TA, Smith PG, Rolfe M
**摘要**：研究报道了MLN4924(NEDD8激活酶抑制剂)的抗癌机制，利用NEDD8抗体验证药物对Cullin蛋白neddylation的抑制作用，证实阻断NEDD8通路可抑制肿瘤生长。
3. **文献名称**：*Detection of protein modification by NEDD8 with specific antibodies*
**作者**：Xirodimas DP, Saville MK, Bourdon JC, Hay RT, Lane DP
**摘要**：开发了高特异性的NEDD8抗体，可区分NEDD8与泛素修饰，揭示p53等蛋白的neddylation动态变化，为研究其生理功能提供工具。
4. **文献名称**：*Structural basis for the activation of the NEDD8 conjugation pathway by the APPBP1-UBA3 complex*
**作者**：Enchev RI, Schulman BA, Peter M
**摘要**：通过结构生物学解析NEDD8激活酶的分子机制，使用抗体验证复合体形成对NEDD8结合的关键作用，阐明neddylation通路的调控机制。
NEDD8 (Neural precursor cell-expressed developmentally downregulated 8) is a ubiquitin-like protein that plays a critical role in post-translational protein modification through a process called neddylation. This covalent conjugation of NEDD8 to target proteins, primarily cullin family members, regulates the activity of Cullin-RING E3 ubiquitin ligases (CRLs), which are essential for ubiquitination and subsequent proteasomal degradation of substrate proteins. NEDD8 shares ~60% sequence identity with ubiquitin but has distinct biochemical pathways mediated by specific E1 (activating), E2 (conjugating), and E3 (ligating) enzymes.
NEDD8 antibodies are vital tools for studying neddylation dynamics in cellular processes such as cell cycle progression, DNA repair, and stress responses. Dysregulation of neddylation is implicated in cancer, neurodegenerative diseases, and viral infections, driving interest in therapeutic targeting. Researchers use NEDD8-specific antibodies to detect neddylated proteins via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies must exhibit high specificity to differentiate NEDD8 from ubiquitin and other ubiquitin-like modifiers, given their structural similarities. Recent applications include investigating MLN4924 (a NEDD8-activating enzyme inhibitor) in preclinical cancer studies and exploring neddylation's role in protein homeostasis. Validation of antibody specificity using knockdown/knockout controls or recombinant proteins remains crucial for experimental accuracy.
×
