| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NEDD-8 |
| Entrez GeneID | 4738 |
| clone | 5B8 |
| WB Predicted band size | 9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NEDD8 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于NEDD8抗体的参考文献及其摘要概述：
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1. **文献名称**: *"A highly specific antibody for detecting endogenous NEDD8 conjugates in vitro and in vivo"*
**作者**: Rabut G. et al.
**摘要**: 该文献报道了一种高特异性的兔源单克隆抗体，可识别内源性NEDD8修饰蛋白，但不与其他泛素样蛋白(如SUMO或ISG15)发生交叉反应。通过Western blot和免疫荧光验证其在多种细胞系和动物模型中的应用，证实该抗体在检测neddylation修饰动态变化中的可靠性。
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2. **文献名称**: *"Development of a monoclonal antibody targeting NEDD8 for cancer therapeutic research"*
**作者**: Zhao Y. et al.
**摘要**: 研究团队开发了一种靶向NEDD8的人源化单克隆抗体，用于抑制neddylation通路在肿瘤细胞中的异常激活。实验表明，该抗体可阻断Cullin蛋白的NEDD8修饰，导致CRL复合物功能抑制，从而诱导肿瘤细胞凋亡，为癌症治疗提供了潜在工具。
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3. **文献名称**: *"NEDD8 antibody-based proximity labeling reveals substrate networks of the NEDD8 conjugation machinery"*
**作者**: Enchev R.I. et al.
**摘要**: 利用NEDD8特异性抗体结合邻近标记技术，系统鉴定了细胞内NEDD8结合酶(如UBA3-APPBP1复合物)的底物网络。该研究揭示了多种新型neddylation靶蛋白，并阐明了其在细胞周期调控和DNA损伤应答中的功能。
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如需更多文献或特定研究方向的补充，请进一步说明需求。
NEDD8 (Neural precursor cell-expressed developmentally downregulated protein 8) is a ubiquitin-like protein that plays a critical role in post-translational protein modification through a process called neddylation. This pathway involves the covalent conjugation of NEDD8 to substrate proteins, primarily members of the cullin family, which are scaffold components of Cullin-RING ubiquitin ligases (CRLs). Neddylation regulates CRL activity, enabling substrate recognition and polyubiquitination for proteasomal degradation. Dysregulation of NEDD8-mediated processes is implicated in cancer, neurodegenerative diseases, and immune disorders.
NEDD8 antibodies are essential tools for studying neddylation dynamics. They detect endogenous NEDD8. its conjugated forms, or neddylation-related enzymes (e.g., NAE, UBE2M/F) in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specificity is crucial due to NEDD8’s high homology (~60%) with ubiquitin and other ubiquitin-like proteins (e.g., SUMO). High-quality antibodies distinguish free NEDD8 from conjugated forms and avoid cross-reactivity, often validated using knockout cell lines or competition assays.
Research applications include investigating CRL-mediated protein turnover, DNA damage responses, and cell cycle regulation. Neddylation inhibitors (e.g., MLN4924) are under clinical evaluation as anticancer agents, driving demand for reliable NEDD8 antibodies to assess drug efficacy and mechanism. These antibodies also aid in exploring pathological neddylation in diseases like Alzheimer’s and viral infections. Continued development of isoform-specific and modification-state-selective antibodies remains vital for dissecting NEDD8’s multifaceted roles.
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