| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ANOP3; MCOPS3 |
| Entrez GeneID | 6657 |
| clone | 10F10 |
| WB Predicted band size | 34kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit,Monkey |
| Immunogen | Purified recombinant fragment of human SOX2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SOX2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Multipotent cell lineages in early mouse development depend on SOX2 function*
**作者**：Avilion, A.A. et al.
**摘要**：该研究首次克隆了小鼠SOX2基因，并利用特异性抗体证实SOX2在胚胎干细胞和内细胞团中的表达，揭示了其对维持多能性和早期胚胎发育的关键作用。
2. **文献名称**：*SOX2 is an oncogene activated by recurrent 3q26.3 amplifications in human lung squamous cell carcinomas*
**作者**：Bass, A.J. et al.
**摘要**：通过免疫组织化学(SOX2抗体)和基因组分析，研究发现SOX2在肺鳞癌中异常高表达，且其扩增与肿瘤发生及患者不良预后显著相关，提示其作为治疗靶点的潜力。
3. **文献名称**：*SOX2 antibody specificity in pluripotent stem cells and cancer: A comparative study*
**作者**：Bergsland, M. et al.
**摘要**：比较了多种商业化SOX2抗体在胚胎干细胞、诱导多能干细胞(iPSCs)及肿瘤组织中的特异性，发现部分抗体存在交叉反应，强调选择高特异性抗体对实验可靠性至关重要。
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以上文献涵盖SOX2在发育生物学、癌症研究及抗体技术评估中的应用，均为领域内经典或高影响力研究。
**Background of SOX2 Antibody**
SOX2 (SRY-related HMG-box 2) is a transcription factor critical for maintaining pluripotency and self-renewal in embryonic stem cells and neural progenitor cells. It belongs to the SOX gene family, characterized by a conserved high-mobility group (HMG) domain that binds DNA to regulate gene expression. SOX2 plays essential roles in early embryogenesis, neurodevelopment, and cellular reprogramming. Dysregulation of SOX2 is linked to developmental disorders and cancers, including glioblastoma, lung cancer, and squamous cell carcinomas, where it often acts as an oncogene.
SOX2 antibodies are immunological tools designed to detect and study the expression, localization, and function of SOX2 protein in research and diagnostics. These antibodies are widely used in techniques like immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and flow cytometry. They aid in identifying SOX2-expressing cells, such as cancer stem cells or pluripotent stem cells, and assessing its role in tumor progression or tissue regeneration.
Validating SOX2 antibodies requires attention to specificity due to potential cross-reactivity with other SOX family members (e.g., SOX1. SOX3). High-quality antibodies are often verified using knockout cell lines or siRNA-mediated silencing. Commercial SOX2 antibodies are typically raised in rabbits or mice against epitopes within the N-terminal transactivation or C-terminal regulatory regions. Their applications span developmental biology, cancer research, and regenerative medicine, providing insights into SOX2’s dual roles in normal physiology and disease.
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